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RecombinantRabMAb

Recombinant Anti-LAMC2 antibody [EPR23654-127] (ab274376)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
  • Western blot - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
  • Flow Cytometry (Intracellular) - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
  • Immunoprecipitation - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
  • Anti-LAMC2 antibody [EPR23654-127] (ab274376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23654-127] to LAMC2
  • Suitable for: IP, Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-LAMC2 antibody [EPR23654-127]
    See all LAMC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23654-127] to LAMC2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cyt (Intra), WB, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 whole cell lysate. ICC/IF: A431 cells. Flow Cyt (intra): A549 cells. IP: A431 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23654-127

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab274376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/30.
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 80, 100, 140 kDa (predicted molecular weight: 131 kDa).
ICC/IF
1/100.
Notes
IP
1/30.
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 80, 100, 140 kDa (predicted molecular weight: 131 kDa).
ICC/IF
1/100.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.

  • Tissue specificity

    The large variant is expressed only in specific epithelial cells of embryonic and neonatal tissues. In 17-week old embryo the small variant is found in cerebral cortex, lung, and distal tubes of kidney, but not in epithelia except for distal tubuli.

  • Involvement in disease

    Defects in LAMC2 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.

  • Sequence similarities

    Contains 8 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.

  • Domain

    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain IV is globular.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt

  • Database links

    • Alternative names

      • 3918 antibody
      • B2T antibody
      • BM600 antibody
      • Cell-scattering factor 140 kDa subunit antibody
      • CSF 140 kDa subunit antibody
      • CSF antibody
      • EBR2 antibody
      • EBR2A antibody
      • Epiligrin subunit gamma antibody
      • Kalinin subunit gamma antibody
      • Kalinin/nicein/epiligrin 100 kDa subunit antibody
      • Ladsin 140 kDa subunit antibody
      • LAMB2T antibody
      • LAMC2 antibody
      • LAMC2_HUMAN antibody
      • Laminin 5 gamma 2 subunit antibody
      • Laminin B2t chain antibody
      • Laminin gamma 2 antibody
      • laminin gamma 2 chain antibody
      • Laminin subunit gamma-2 antibody
      • Laminin-5 subunit gamma antibody
      • LAMNB2 antibody
      • Large adhesive scatter factor 140 kDa subunit antibody
      • MGC138491 antibody
      • MGC141938 antibody
      • Nicein subunit gamma antibody
      • NICEIN-100KDA antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
      Immunocytochemistry/ Immunofluorescence - Anti-LAMC2 antibody [EPR23654-127] (ab274376)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling LAMC2 with ab274376 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A431 cell line. Low expression control: A549 (PMID: 25591736). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200  dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Western blot - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
      Western blot - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
      All lanes : Anti-LAMC2 antibody [EPR23654-127] (ab274376) at 1/1000 dilution

      Lane 1 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
      Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate
      Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 131 kDa
      Observed band size: 140,80 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Bands of 140KDa and 80KDa respectively represent  the full length and endogenously cleaved form.

      The expression profile observed is consistent with what has been described in the literature (PMID: 25591736, 21829200).

      Low expression: A549 (PMID:25591736) and WI-38 (PMID: 10964684)

      Expsure time: 3 minutes

       

    • Flow Cytometry (Intracellular) - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
      Flow Cytometry (Intracellular) - Anti-LAMC2 antibody [EPR23654-127] (ab274376)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell) (Left) A431 (Human epidermoid carcinoma epithelial cell) (Right) cells labelling LAMC2 with ab274376 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      Low expression control: A549. 

       

       

    • Immunoprecipitation - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
      Immunoprecipitation - Anti-LAMC2 antibody [EPR23654-127] (ab274376)

      LAMC2 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell), whole cell lysate with ab274376 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274376 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate 10 ug

      Lane 2: ab274376 IP in A431 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab274376 in A431 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 41 seconds

      Band of 140KDa represents the full length form, Bands of 100KDa and 80KDa represent the endogenously cleaved form.

      The expression profile observed is consistent with what has been described in the literature (PMID: 21829200).

    • Anti-LAMC2 antibody [EPR23654-127] (ab274376)
      Anti-LAMC2 antibody [EPR23654-127] (ab274376)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab274376? Please let us know so that we can cite the reference in this datasheet.

    ab274376 has been referenced in 1 publication.

    • You X  et al. Galectin-1 promotes vasculogenic mimicry in gastric adenocarcinoma via the Hedgehog/GLI signaling pathway. Aging (Albany NY) 12:21837-21853 (2020). PubMed: 33170154

    Customer reviews and Q&As

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