Name: Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]
Brand: RabMAb
SKU: ab108922
Rating: 5 (6 reviews)

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Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C
Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

Product name

Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]
See all Lamin A+Lamin B1+Lamin C primary antibodies
Description

Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C
Host species

Rabbit
Specificity

The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.
Tested applications

Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Spermophilus tridecemlineatus
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse heart and rat heart tissue lysates and HeLa, HepG2 and HACAT cell lysates, ab83472. IHC-P: Human ovarian carcinoma, human liver carcinoma, human breast, human uterus, human kidney mouse liver and rat liver tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HepG2 lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4068
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- Lamins
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- Lamins

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Hep G2 nuclear extract lysate (ab14660)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/100 - 1/250.
Flow Cyt (Intra)		1/20.
WB	(4)	1/1000 - 1/10000. Predicted molecular weight: 74 kDa.
IP		1/10 - 1/100.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
ICC/IF 1/100 - 1/250.
Flow Cyt (Intra) 1/20.
WB 1/1000 - 1/10000. Predicted molecular weight: 74 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Cellular localization

Lamin A: Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C. Lamin B1: Nucleus inner membrane. Lamin C: Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C.
Database links
- Entrez Gene: 4000 Human
- Entrez Gene: 4001 Human
- Entrez Gene: 16905 Mouse
- Entrez Gene: 16906 Mouse
- Entrez Gene: 116685 Rat
- Entrez Gene: 60374 Rat
- Omim: 150330 Human
- Omim: 150340 Human
- SwissProt: P02545 Human
- SwissProt: P20700 Human
- SwissProt: P14733 Mouse
- SwissProt: P48678 Mouse
- SwissProt: P48679 Rat
- SwissProt: P70615 Rat
- Unigene: 594444 Human
- Unigene: 89497 Human
- Unigene: 243014 Mouse
- Unigene: 4105 Mouse
- Unigene: 471227 Mouse
- Unigene: 11362 Rat
- Unigene: 44161 Rat
see all
Form

Lamin C: There are 3 isoforms produced by alternative splicing. Isoform A also known as Lamin A; Isoform C also known as Lamin C; Isoform ADelta10 also known as Lamin ADelta10.

Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

All lanes : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/10000 dilution (purified)

Lane 1 : mouse heart lysate
Lane 2 : rat heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

Predicted band size: 74 kDa
Additional bands at: 65 kDa (possible isoform), 70 kDa (possible isoform), 74 kDa (possible isoform)

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunofluorescence staining of HeLa cells with purified ab108922 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108922 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunohistochemical staining of paraffin embedded rat liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

All lanes : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/1000 dilution

Lane 1 : Recombinant Human Lamin A protein (ab83472)
Lane 2 : GST tagged recombinant Human Lamin B1 protein (1 to 586) (91 KDa)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 75, 91 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking/Diluting buffer and concentration 5% NFDM/TBST
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Lane 1 : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/1000 dilution
Lane 2 : Anti-Myc tag antibody at 1/10000 dilution
Lane 3 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/5000 dilution

All lanes : Myc and DDK tagged recombinant Human Lamin B2 protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?

Exposure time: 7 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

ab108922 does not cross react with Lamin B2.
Flow Cytometry (Intracellular) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Intracellular Flow Cytometry analysis of HeLa cells labelling Lamin A + B1 + C with purified ab108922 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour^®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunoprecipitation - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

ab108922 (purified) at 1/20 immunoprecipitating Lamin A + B1 + C in 10 μg HepG2 (Lanes 1 and 2, observed at 70, 74, and 65 kDa - ab108922 recognises three isoforms). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking buffer and concentration: 5% NFDM/TBST.

Dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/10000 dilution (purified) + HeLa cell lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

Predicted band size: 74 kDa
Additional bands at: 65 kDa (possible isoform), 70 kDa (possible isoform), 74 kDa (possible isoform)

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunohistochemical staining of paraffin embedded human liver carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunohistochemical staining of paraffin embedded mouse liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Immunohistochemical analysis of paraffin-embedded human colonic tissue using unpurified ab108922 at a diltion of 1/100
Western blot - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

All lanes : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HACAT cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 74 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Unpurified ab108922 showing positive staining in Normal human breast tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Unpurified ab108922 showing positive staining in Normal human uterus tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Unpurified ab108922 showing positive staining in Normal human kidney tissue.
Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab108922? Please let us know so that we can cite the reference in this datasheet.

ab108922 has been referenced in 29 publications.

Zhou C & Homer HA The oocyte spindle midzone pauses Cdk1 inactivation during fertilization to enable male pronuclear formation and embryo development. Cell Rep 39:110789 (2022). PubMed: 35508138
Knox J et al. MiR-18a-5p Targets Connective Tissue Growth Factor Expression and Inhibits Transforming Growth Factor β2-Induced Trabecular Meshwork Cell Contractility. Genes (Basel) 13:N/A (2022). PubMed: 36011411
Sui H et al. Manganese enhances DNA- or RNA-mediated innate immune response by inducing phosphorylation of TANK-binding kinase 1. iScience 25:105352 (2022). PubMed: 36325059
Sharma BK et al. Fibrinogen activates focal adhesion kinase (FAK) promoting colorectal adenocarcinoma growth. J Thromb Haemost 19:2480-2494 (2021). PubMed: 34192410
Fan G et al. The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation. Nat Commun 12:20 (2021). PubMed: 33397932

View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Application

ChIP

Sample

Human Cell lysate - nuclear (K562)

Negative control

IgG

Specification

K562

Detection step

Other

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Andrew Perez

Verified customer

Submitted Mar 24 2022

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Macrophage Cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

Macrophage Cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 17 2018

Application

Flow Cytometry

Sample

Mouse Cell (Mouse embryonic fibroblasts (wild type and LMNA kn)

Permeabilization

Yes - 0.1% saponin

Specification

Mouse embryonic fibroblasts (wild type and LMNA kn

Fixation

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 18 2017

Application

Western blot

Sample

Human Cell lysate - nuclear (HEK293 Lysate (kidney cell))

Gel Running Conditions

Reduced Denaturing (4-12% Gradient)

Loading amount

5 µg

Specification

HEK293 Lysate (kidney cell)

Blocking step

Licor Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Sam Nowitzki

Verified customer

Submitted Jul 28 2015

Application

Western blot

Sample

Human Cell lysate - nuclear (HEK nuclear and cytoplasmic lysates)

Gel Running Conditions

Reduced Denaturing

Loading amount

15 µg

Specification

HEK nuclear and cytoplasmic lysates

Blocking step

Licor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 08 2015

Application

Western blot

Sample

Spermophilus tridecemlineatus Cell lysate - nuclear (Brown adipose tissue)

Gel Running Conditions

Reduced Denaturing (10% Tris-Glycine)

Loading amount

30 µg

Specification

Brown adipose tissue

Blocking step

BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. James Bjork

Verified customer

Submitted Dec 19 2012

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Cellular localization

Database links

Form

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (29)

Customer reviews and Q&As

ChIP abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

Western blot abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

Flow Cytometry abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

Western blot abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

Western blot abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

Western blot abreview for Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]