Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)
![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)](https://www.abcam.com/ps/products/8/ab8982/Images/ab8982-281492-anti-lamin-b1-antibody-119d5-f1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)")
Key features and details
- Mouse monoclonal [119D5-F1] to Lamin B1 - Nuclear Envelope Marker
- Suitable for: Flow Cyt, ICC/IF, IHC-Fr
- Knockout validated
- Reacts with: Mouse, Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker
See all Lamin B1 primary antibodies -
Description
Mouse monoclonal [119D5-F1] to Lamin B1 - Nuclear Envelope Marker -
Host species
Mouse -
Specificity
Reacts with an epitope located C-terminal of residue 231 in lamin B1. Reacts against lamin B1, does not cross react with lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. ab8982 has been shown to react with HeLa and 3T3 cells in immunocytochemistry. Other cell/tissue types have not been tested. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human, Pig -
Immunogen
Full length native protein (purified) corresponding to Rat Lamin B1 (C terminal). Immunogen is purified rat liver lamins.
Database link: P20700 -
Epitope
C-terminal to residue 231. -
Positive control
- ICC/IF: HAP1, HeLa and 3T3 cells. WB: Human mammary cell lysate. IHC-Fr: Human kidney and colon tissue; MCF7 cell culture; Swine liver tissue. Flow Cyt: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
119D5-F1 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/100 - 1/200.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
Use a concentration of 0.5 µg/ml.
|
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| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
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Flow Cyt
1/100 - 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use a concentration of 0.5 µg/ml. |
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IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
Involvement in disease
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsB-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
Cellular localization
Nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4001 Human
- Entrez Gene: 16906 Mouse
- Entrez Gene: 100513342 Pig
- Omim: 150340 Human
- SwissProt: P20700 Human
- SwissProt: P14733 Mouse
- Unigene: 89497 Human
- Unigene: 4105 Mouse
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Alternative names
- ADLD antibody
- lamin B1 antibody
- Lamin-B1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)
ab8982 staining Lamin B1 in wild-type HAP1 cells (top panel) and LMNB1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8982 at 0.5μg/ml and ab202272 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)](https://www.abcam.com/ps/products/8/ab8982/Images/ab8982-391627-anti-lamin-b1-antibody-119d5-f1-nuclear-envelope-marker-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)Immunohistochemistry on frozen sections of human kidney showing nuclear lamina staining in epithelial and connective tissue cells.
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![Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)](https://www.abcam.com/ps/products/8/ab8982/Images/ab8982-391626-anti-lamin-b1-antibody-119d5-f1-nuclear-envelope-marker-immunohistochemistry-pig-liver.jpg)
Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)Immunohistochemistry of MCF-7 cell culture showing nuclear lamina staining.
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![Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)](https://www.abcam.com/ps/products/8/ab8982/Images/ab8982-15963-anti-lamin-b1-antibody-119d5-f1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)This image is courtesy of Marilena Ciciarello & Patrizia Lavia, University La SapienzaMethanol fixed HeLa and 3T3 cells (ab7179) were stained with ab8982 (1/50 and 1/20 respectively). The cells were fixed in 100% methanol for 6 minutes at -20°C. ab8982 clearly stains the nuclear envelope with very little background staining.
A: HeLa cells + ab8982 (1/50) (green)
B: HeLa cells counterstained with DAPI (blue)
C. 3T3 cells + ab8982 (1/20) (green)
D. 3T3 cells counterstained with DAPI (blue) -
![Flow Cytometry - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)](https://www.abcam.com/ps/products/8/ab8982/Images/ab8982-15962-anti-lamin-b1-antibody-119d5-f1-flow-cytometry.jpg)
Flow Cytometry - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)Overlay histogram showing HeLa cells stained with ab8982 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8982, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)](https://www.abcam.com/ps/products/8/ab8982/Images/ab8982-15958-anti-lamin-b1-antibody-119d5-f1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)Immunohistochemistry on frozen sections of swine liver showing nuclear lamina staining in hepatocytes.
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![Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)](https://www.abcam.com/ps/products/8/ab8982/Images/ab8982-15959-anti-lamin-b1-antibody-119d5-f1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab8982 has been referenced in 40 publications.
- Meharena HS et al. Down-syndrome-induced senescence disrupts the nuclear architecture of neural progenitors. Cell Stem Cell 29:116-130.e7 (2022). PubMed: 34995493
- Muralidharan H et al. KIFC1 Regulates the Trajectory of Neuronal Migration. J Neurosci 42:2149-2165 (2022). PubMed: 35046122
- Wang YF et al. Seawater immersion exacerbates the pathological changes caused by incisive corneal injury in rabbit eyes. Ann Transl Med 10:589 (2022). PubMed: 35722388
- Zhou CJ et al. CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I. Nat Commun 13:7732 (2022). PubMed: 36513638
- Iyer KV et al. Apico-basal cell compression regulates Lamin A/C levels in epithelial tissues. Nat Commun 12:1756 (2021). PubMed: 33767161