Anti-Lamin B1 antibody (ab65986)
Key features and details
- Rabbit polyclonal to Lamin B1
- Suitable for: IHC-Fr, WB, IHC-P, ICC
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Lamin B1 antibody
See all Lamin B1 primary antibodies -
Description
Rabbit polyclonal to Lamin B1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Lamin B1 aa 570-586 (C terminal).
Sequence:FHQQGTPRASNRSCAIM
Database link: P20700 -
Positive control
- WB: Rat brain tissue lysate IHC-P:Mammary cancer sections; IHC-F: Rat Intestine Tissue ICC: A549 Cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml. Formalin or acetone fixed tissues.
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| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
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| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC |
Use a concentration of 0.5 - 1 µg/ml. Acetone fixed.
|
| Notes |
|---|
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IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. Formalin or acetone fixed tissues. |
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa). |
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC
Use a concentration of 0.5 - 1 µg/ml. Acetone fixed. |
Target
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Function
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
Involvement in disease
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsB-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
Cellular localization
Nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 4001 Human
- Entrez Gene: 116685 Rat
- Omim: 150340 Human
- SwissProt: P20700 Human
- SwissProt: P70615 Rat
- Unigene: 89497 Human
- Unigene: 11362 Rat
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Alternative names
- ADLD antibody
- lamin B1 antibody
- Lamin-B1 antibody
see all
Images
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Western blot - Anti-Lamin B1 antibody (ab65986)All lanes : Anti-Lamin B1 antibody (ab65986)
Lane 1 : MCF-7 Cell lysate
Lane 2 : HeLa Cell lysate
Predicted band size: 66 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody (ab65986) -
Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody (ab65986)Immunohistochemistry (Frozen sections) of Rat Intestine Tissue staining Lamin B1 with ab65986
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Immunocytochemistry - Anti-Lamin B1 antibody (ab65986)Immunocytochemistry of A549 Cells staining Lamin B1 with ab65986.
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Western blot - Anti-Lamin B1 antibody (ab65986)Anti-Lamin B1 antibody (ab65986) at 1 µg/ml + rat brain tissue lysate
Predicted band size: 66 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab65986 has been referenced in 39 publications.
- Ke B et al. APC ameliorates idiopathic membranous nephropathy by affecting podocyte apoptosis through the ERK1/2/YB-1/PLA2R1 axis. Mol Cell Biochem N/A:N/A (2023). PubMed: 36588134
- Lotlikar MS et al. Microfluidic separation of axonal and somal compartments of neural progenitor cells differentiated in a 3D matrix. STAR Protoc 3:101028 (2022). PubMed: 35059649
- Ni H et al. Cardamonin attenuates cerebral ischemia/reperfusion injury by activating the HIF-1α/VEGFA pathway. Phytother Res 36:1736-1747 (2022). PubMed: 35142404
- Lee SH et al. Povidone iodine suppresses LPS-induced inflammation by inhibiting TLR4/MyD88 formation in airway epithelial cells. Sci Rep 12:3681 (2022). PubMed: 35256715
- Rivera C et al. Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatin. Nat Commun 13:1550 (2022). PubMed: 35322029