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    products/primary-antibodies/lamin-b1-antibody-epr8985b-nuclear-envelope-marker-ab133741.pdf

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Cell Biology Apoptosis Nucleus Lamins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)

  • Datasheet
  • SDS
Reviews (14)Q&A (1)References (126)

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Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
  • Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8985(B)] to Lamin B1 - Nuclear Envelope Marker
  • Suitable for: IP, ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
Knockout
Product image
Human LMNB1 (Lamin B1) knockout HeLa cell line (ab255404)

View more associated products

Overview

  • Product name

    Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker
    See all Lamin B1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8985(B)] to Lamin B1 - Nuclear Envelope Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. within Human Lamin B1 aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: P20700

  • Positive control

    • WB: Hap1, HeLa, Jurkat, Molt4, Y79, Caco 2, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates. Mouse brain,heart, kidney and spleen; and Rat brain, heart and spleen lysates. IHC-P: Human colon, liver and transitional cell carcinoma of the bladder tissues. ICC/IF: Ramos cells, HAP1-LMNB1 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.95 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8985(B)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Lamins
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class V
    • Lamins
    • Isotype/Loading Controls
    • Loading Controls
    • Lamin B1 - Nuclear
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Lamins

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
    • Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)
    • HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)
    • Alexa Fluor® 594 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab216723)
    • Anti-Lamin B1 antibody [EPR8985(B)] - BSA and Azide free (ab220797)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human LMNB1 (Lamin B1) knockout HeLa cell line (ab255404)
  • KO cell lysates

    • Human LMNB1 (Lamin B1) knockout HeLa cell lysate (ab263825)
  • Positive Controls

    • NIH/3T3 nuclear extract lysate (ab14874)
    • PC-12 nuclear extract lysate (ab14884)
    • NIH/3T3 whole cell lysate (ab7179)
    • MOLT-4 whole cell lysate (ab7912)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/20.
ICC/IF (4)
Use a concentration of 1 µg/ml.
WB (9)
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
IHC-P (1)
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
IP
1/20.
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
IHC-P
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in disease

    Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Post-translational
    modifications

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localization

    Nucleus inner membrane.
  • Target information above from: UniProt accession P20700 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4001 Human
    • Entrez Gene: 16906 Mouse
    • Entrez Gene: 116685 Rat
    • Omim: 150340 Human
    • SwissProt: P20700 Human
    • SwissProt: P14733 Mouse
    • SwissProt: P70615 Rat
    • Unigene: 89497 Human
    • Unigene: 4105 Mouse
    • Unigene: 11362 Rat
    see all
  • Alternative names

    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Images

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    All lanes : Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : LMNB1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 66-70 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab133741 observed at 66-70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab133741 was shown to react with LMNB1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255404 (knockout cell lysate ab263825) was used. Wild-type HeLa and LMNB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133741 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (Human Burkitt's lymphoma cell line) cells labeling Lamin B1 with ab133741 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/200 dilution (green). Nuclear envelope staining on Ramos cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab133741 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/200 dilution.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of the bladder tissue labeling Lamin B1 with purified ab133741 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)

    ab133741 staining Lamin B1 in wild-type HAP1 cells (top panel) and Lamin B1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab133741 at 1μg/ml dilution and ab195889 at 1/250 dilution (shown in pseudo-color red) overnight at +4°C. The cells were then incubated with ab150081 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)) at 1/1000 dilution for 1 hour. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Lanes 1 & 3 : Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/1000 dilution


    Lane 1 : Wild-type HAP1 whole cell lysate at 20 µg
    Lanes 2 & 4 : Empty
    Lane 3 : LMNB1 knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 66 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab133741 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133741 was shown to specifically react with Lamin B1 in wild type HAP1 cells. No band was observed when knockout samples were used. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. Ab133741 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Lanes 1 & 3 : Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)


    Lane 1 : Wild-type HAP1 whole cell lysate at 20 µg
    Lane 2 : Empty
    Lane 3 : Lamin B1 knockout HAP1 cell lysate at 20 µg

    Predicted band size: 66 kDa



    Lanes 1 - 3: Merged signal (red and green).

    Green - Target observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab133741 and a competitor's discontinued goat polyclonal antibody.

     

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/1000 dilution + GST-tagged Recombinant Human Lamin B1 protein (aa 1 to 586) at 0.015 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Recombinant Human Lamin B1 protein (ab114163)

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    All lanes : Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/50000 dilution (purified)

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood)
    cell lysate
    Lane 2 : Molt-4 (Human lymphoblastic leukemia cell line) cell lysate
    Lane 3 : Y79 (Human retinoblastoma cell line) cell lysate
    Lane 4 : Caco-2 (Human colorectal adenocarcinoma cells) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)

    Immunohistochemical staining of paraffin embedded Mouse Cerebral cortex with purified ab133741 at a working dilution of 1/300. The secondary antibody used is a HRP polymer for rabbit IgG. Nuclear envelope staining on neuron cells of Cerebral cortex tissue is observed. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    All lanes : Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/50000 dilution (purified)

    Lane 1 : C6 (Rat glial tumor cells) cell lysate
    Lane 2 : PC12 (Rat adrenal gland pheochromocytoma) cell lysate
    Lane 3 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate
    Lane 4 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/10000 dilution (purified) + Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µg

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Lamin B1 with unpurified ab133741 at 1/250.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    OI-RD Scanning - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Western blot - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    All lanes : Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) at 1/10000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Mouse spleen lysates
    Lane 5 : Rat brain lysates
    Lane 6 : Rat heart lysates
    Lane 7 : Rat spleen lysates
    Lane 8 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 10 : NIH/3T3 (Mouse embyro fibroblast cells)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lamin B1 with unpurified ab133741 at 1/250.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Immunoprecipitation - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)

    ab133741 (purified) at 1/20 immunoprecipitating Lamin B1 in Jurkat cells (Lane 1).  For western blotting, ab133741 was used at 1/1000 dilution and an HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)
    Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741)

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (126)

Publishing research using ab133741? Please let us know so that we can cite the reference in this datasheet.

ab133741 has been referenced in 126 publications.

  • Yang L  et al. Effect of the isoflavone corylin from cullen corylifolium on colorectal cancer growth, by targeting the STAT3 signaling pathway. Phytomedicine 80:153366 (2021). PubMed: 33080566
  • Kim S  et al. Characterization of ferroptosis in kidney tubular cell death under diabetic conditions. Cell Death Dis 12:160 (2021). PubMed: 33558472
  • Chen D  et al. Downregulation of long non-coding RNA MR4435-2HG suppresses breast cancer progression via the Wnt/ß-catenin signaling pathway. Oncol Lett 21:373 (2021). PubMed: 33777197
  • Kang JW  et al. PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells. Signal Transduct Target Ther 6:129 (2021). PubMed: 33785736
  • Todkar K  et al. Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs. Nat Commun 12:1971 (2021). PubMed: 33785738
View all Publications for this product

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Intestine, spleen, liver.)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9
Permeabilization
No
Specification
Intestine, spleen, liver.
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Victor Bernal

Verified customer

Submitted Apr 23 2018

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