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RecombinantRabMAb

Recombinant Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

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Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
  • Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
  • Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
  • Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
  • Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9701(B)] to Lamin B2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC HRP PE Unconjugated

Overview

  • Product name

    Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free
    See all Lamin B2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9701(B)] to Lamin B2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HepG2 cell lysate; IHC: Human bladder cancer tissue; ICC/IF: HepG2 cells; WB: HepG2 and HeLa cells, Mouse and rat testis and rat spleen lysates. Flow Cyt (intra): HepG2 cells.

  • General notes

    The human and rat recommendation is based on the WB results. We do not guarantee IHC-P for human and rat.

    ab240124 is the carrier-free version of ab151735.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The human and rat recommendation is based on the WB results. We do not guarantee IHC-P for human and rat.
WB
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The human and rat recommendation is based on the WB results. We do not guarantee IHC-P for human and rat.
WB
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.

Target

  • Function

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

  • Involvement in disease

    Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

  • Cellular localization

    Nucleus inner membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • LAMB 2 antibody
      • LAMB2 antibody
      • Lamin-B2 antibody
      • LMN 2 antibody
      • LMN B2 antibody
      • LMN2 antibody
      • LMNB 2 antibody
      • LMNB2 antibody
      • LMNB2_HUMAN antibody
      • MGC2721 antibody
      • RGD1563803 antibody
      see all

    Images

    • Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
      Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
      All lanes : Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : Mouse testis lysate
      Lane 3 : Rat spleen lysate
      Lane 4 : Rat testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 68 kDa
      Observed band size: 68 kDa



      This data was developed using ab151735, the same antibody clone in a different buffer formulation. 

      Blocking Buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
      Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
      Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 68 kDa
      Observed band size: 68 kDa



      This data was developed using ab151735, the same antibody clone in a different buffer formulation. 

      Blocking Buffer and concentration: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
      Immunocytochemistry/ Immunofluorescence - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

      This data was developed using ab151735, the same antibody clone in a different buffer formulation.
      Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab240124 at 1:50 dilution (2.18 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
      Flow Cytometry (Intracellular) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

      This data was developed using ab151735, the same antibody clone in a different buffer formulation.Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab240124 at 1/20 dilution (10µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

      This data was developed using ab151735, the same antibody clone in a different buffer formulation.
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder cancer tissue sections labeling Lamin B2 with Purified ab240124 at 1:100 dilution (1.09 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
      Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

      This data was developed using ab151735, the same antibody clone in a different buffer formulation.

      Purified ab151735 at 1/20 dilution (0.5µg) immunoprecipitating Lamin B2 in HepG2 whole cell lysate.
      Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab151735 + HepG2 whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151735 in HepG2 whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 68 kDa

    • Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
      Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240124? Please let us know so that we can cite the reference in this datasheet.

    ab240124 has not yet been referenced specifically in any publications.

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