Recombinant Anti-Laminin 2 alpha antibody [EPR23924-60] (ab259345)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] (ab259345)](https://www.abcam.com/ps/products/259/ab259345/Images/ab259345-1-anti-laminin-2-alpha-antibody-epr2392460-immunohistochemistry-mouse-cardiac-muscle.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] (ab259345)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23924-60] to Laminin 2 alpha
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
-
Product name
Anti-Laminin 2 alpha antibody [EPR23924-60]
See all Laminin 2 alpha primary antibodies -
Description
Rabbit monoclonal [EPR23924-60] to Laminin 2 alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Mouse cardiac muscle and skeletal muscle tissue. IHC-Fr: Mouse cardiac muscle tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23924-60 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab259345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/100.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-Fr
1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone. -
Involvement in disease
Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI. -
Sequence similarities
Contains 17 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 2 laminin IV type A domains.
Contains 1 laminin N-terminal domain. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI, IV and G are globular. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Major component. - Information by UniProt
-
Database links
- Entrez Gene: 16773 Mouse
- SwissProt: Q60675 Mouse
- Unigene: 256087 Mouse
-
Alternative names
- LAMA 2 antibody
- LAMA2 antibody
- LAMA2_HUMAN antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] (ab259345)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Laminin 2 alpha with ab259345 at 1/500 (0.484 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in basal laminae of mouse cardiac muscle (PMID: 11969289). The section was incubated with ab259345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] (ab259345)](https://www.abcam.com/ps/products/259/ab259345/Images/ab259345-3-anti-laminin-2-alpha-antibody-epr2392460-immunohistochemistry-frozen-mouse-cardiac-muscle.jpg)
Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] (ab259345)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle tissue labeling Laminin 2 alpha with ab259345 at 1/100 (4.84 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Green). Positive staining on mouse cardiac muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] (ab259345)](https://www.abcam.com/ps/products/259/ab259345/Images/ab259345-2-anti-laminin-2-alpha-antibody-epr2392460-immunohistochemistry-mouse-skeletal-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] (ab259345)Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Laminin 2 alpha with ab259345 at 1/500 (0.484 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in basal laminae of mouse skeletal muscle (PMID: 11969289). The section was incubated with ab259345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-Laminin 2 alpha antibody [EPR23924-60] (ab259345)](https://www.abcam.com/ps/products/259/ab259345/Images/ab259345-5-benefits-of-recombinant-antibodies.png)
Anti-Laminin 2 alpha antibody [EPR23924-60] (ab259345)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab259345 has not yet been referenced specifically in any publications.