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  1. Link

    products/primary-antibodies/laminin-antibody-ab11575.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Extracellular Matrix
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Anti-Laminin antibody (ab11575)

  • Datasheet
  • SDS
Reviews (71)Q&A (20)References (458)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)
  • Dot Blot - Anti-Laminin antibody (ab11575)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)

Key features and details

  • Rabbit polyclonal to Laminin
  • Suitable for: Dot blot, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Laminin antibody
    See all Laminin primary antibodies
  • Description

    Rabbit polyclonal to Laminin
  • Host species

    Rabbit
  • Specificity

    In dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C.
  • Tested applications

    Suitable for: Dot blot, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Pig, Xenopus laevis, Reptile, Mammals, Amphibian
  • Immunogen

    Full length native protein (purified) corresponding to Laminin. The immunogen is laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.

  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.027% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
1/1000. This concentration was determined using laminin at 50 ng per dot.
IHC-P (27)
Use at an assay dependent concentration.
Notes
Dot blot
1/1000. This concentration was determined using laminin at 50 ng per dot.
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity

    Broadly expressed in: skin, heart, lung, and the reproductive tracts.
  • Sequence similarities

    Contains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • Domain

    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain IV is globular.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Target information above from: UniProt accession Q9Y6N6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10319 Human
    • Entrez Gene: 284217 Human
    • Entrez Gene: 3908 Human
    • Entrez Gene: 3912 Human
    • Entrez Gene: 3913 Human
    • Entrez Gene: 3915 Human
    • Entrez Gene: 3918 Human
    • Entrez Gene: 16772 Mouse
    • Entrez Gene: 316758 Rat
    • Omim: 150320 Human
    • SwissProt: P07942 Human
    • SwissProt: P24043 Human
    • SwissProt: P25391 Human
    • SwissProt: P55268 Human
    • SwissProt: Q9Y6N6 Human
    • SwissProt: P19137 Mouse
    • SwissProt: Q9R0B6 Mouse
    • Unigene: 201805 Human
    • Unigene: 302362 Mouse
    see all
  • Alternative names

    • LAMA 1 antibody
    • LAMA antibody
    • LAMA1 antibody
    • LAMB1 antibody
    • Lamc3 antibody
    • LAMC3_HUMAN antibody
    • Laminin A chain antibody
    • Laminin alpha 1 antibody
    • Laminin alpha 1 chain antibody
    • Laminin B1 chain antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit gamma-3 antibody
    • Laminin-12 subunit gamma antibody
    • Laminin-14 subunit gamma antibody
    • Laminin-15 subunit gamma antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)
    Immunohistochemical analysis of formalin fixed paraffin-embedded human tongue sections labeling Laminin with ab11575 at 1/30 dilution. Goat Anti-Rabbit IgG, FITC conjugate was used as secondary antibody.
     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)

    Immunohistochemical analysis of formalin fixed paraffin-embedded human tonsil sections labeling Laminin with ab11575 at 1/50 dilution. Goat Anti-Rabbit IgG, Cy3™ conjugate was used as secondary antibody.

  • Dot Blot - Anti-Laminin antibody (ab11575)
    Dot Blot - Anti-Laminin antibody (ab11575)

    Immunoblotting
    Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane was separated on SDS-PAGE and probed with Rabbit Anti-Laminin ab11575. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase secondary antibody and a chemiluminescent substrate.
    Lanes
    1. antibody dilution 1/1,000
    2. antibody dilution 1/2,000
    3. antibody dilution 1/4,000
    4. antibody dilution 1/8,000
    5. antibody dilution 1/16,000
    6. antibody dilution 1/32,000
    7. Negative control: without primary antibody

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)
    This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (458)

Publishing research using ab11575? Please let us know so that we can cite the reference in this datasheet.

ab11575 has been referenced in 458 publications.

  • Singh R  et al. Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix. Stem Cells Transl Med 10:492-505 (2021). PubMed: 33145960
  • Yang S  et al. Cell dynamics in Hertwig's epithelial root sheath are regulated by ß-catenin activity during tooth root development. J Cell Physiol 236:5387-5398 (2021). PubMed: 33377198
  • Simsa R  et al. Brain organoid formation on decellularized porcine brain ECM hydrogels. PLoS One 16:e0245685 (2021). PubMed: 33507989
  • Mohad V  et al. Inactivation of Lats1 and Lats2 highlights the role of hippo pathway effector YAP in larynx and vocal fold epithelium morphogenesis. Dev Biol 473:33-49 (2021). PubMed: 33515576
  • Zbinden A  et al. Nidogen-1 Mitigates Ischemia and Promotes Tissue Survival and Regeneration. Adv Sci (Weinh) 8:2002500 (2021). PubMed: 33643791
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 91 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Laminin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 24 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Laminin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 24 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Laminin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Catshark Tissue sections (Whole embryo section)
Permeabilization
Yes - TritonX100 0.25%
Specification
Whole embryo section
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Rami Reshef

Verified customer

Submitted Sep 13 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Laminin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Kidney)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Kidney
Blocking step
(agent) for 5 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Frazer Bell

Verified customer

Submitted May 16 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Laminin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6 & TRIS pH 9
Permeabilization
Yes - 0.3% Triton X-100
Specification
Colon
Blocking step
Donkey Serum 10% + 3% BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Dylan Windell

Verified customer

Submitted May 05 2022

Immunohistochemistry (Frozen sections) abreview for Anti-Laminin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skeletal muscle)
Permeabilization
Yes - TritonX (0.1%)
Specification
Skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 28 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Laminin antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CitricAcid buffer pH 6.0
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 30 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Laminin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lung)
Permeabilization
Yes - 0.1% Triton
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ziv Raviv

Verified customer

Submitted Apr 05 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Laminin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (muscle)
Permeabilization
Yes - 0.5%triton-x 100
Specification
muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 28 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Laminin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 24 2020

1-10 of 91 Abreviews or Q&A

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