Recombinant Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536)
![Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536)](https://www.abcam.com/ps/products/108/ab108536/Images/ab108536-447936-anti-laminin-beta-1-antibody-epr31892-western-blot-hela-lysates.jpg "Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3189(2)] to Laminin beta 1
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Laminin beta 1 antibody [EPR3189(2)]
See all Laminin beta 1 primary antibodies -
Description
Rabbit monoclonal [EPR3189(2)] to Laminin beta 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, JAR, A431, SW480, 293T and C6 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3189(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 198 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 198 kDa. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Sequence similarities
Contains 13 laminin EGF-like domains.
Contains 1 laminin IV type B domain.
Contains 1 laminin N-terminal domain. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Major component. - Information by UniProt
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Database links
- Entrez Gene: 3912 Human
- Entrez Gene: 16777 Mouse
- Entrez Gene: 298941 Rat
- Omim: 150240 Human
- SwissProt: P07942 Human
- SwissProt: P02469 Mouse
- Unigene: 650585 Human
- Unigene: 172674 Mouse
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Alternative names
- CLM antibody
- Cutis laxa with marfanoid phenotype antibody
- LAM B1 antibody
see all
Images
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Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536)All lanes : Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LAMB1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 198 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab108536 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.
ab108536 was shown to react with Laminin beta 1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265414 (knockout cell lysate ab257499) was used. Wild-type HeLa and LAMB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108536 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536)](https://www.abcam.com/ps/products/108/ab108536/Images/ab108536-121262-anti-laminin-beta-1-antibody-epr31892-western-blot.jpg)
Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536)All lanes : Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : A431 cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 198 kDa -
![Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536)](https://www.abcam.com/ps/products/108/ab108536/Images/ab108536-2-benefits-of-recombinant-antibodies.png)
Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab108536 has been referenced in 2 publications.
- McKay TB et al. Corneal Epithelial-Stromal Fibroblast Constructs to Study Cell-Cell Communication in Vitro. Bioengineering (Basel) 6:N/A (2019). PubMed: 31817298
- Bacci L et al. H19-Dependent Transcriptional Regulation of ß3 and ß4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer. Int J Mol Sci 20:N/A (2019). PubMed: 31426484