Recombinant Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)](https://www.abcam.com/ps/products/233/ab233389/Images/ab233389-309870-anti-laminin-gamma-1-antibody-epr21199-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21199] to Laminin gamma 1
- Suitable for: WB, IHC-P, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Laminin gamma 1 antibody [EPR21199]
See all Laminin gamma 1 primary antibodies -
Description
Rabbit monoclonal [EPR21199] to Laminin gamma 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431, NIH:OVCAR-3, HeLa, MCF7, C6, PC-12 and A549 whole cell lysates; Human fetal heart and fetal kidney tissue lysates; rat kidney, brain and spleen tissue lysates; mouse brain tissue lysate. IP: A431 whole cell lysate. IHC-P: Human pancreas tissue; mouse kidney tissue; rat stomach tissue. Flow Cyt (intra): A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21199 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 210-250 kDa (predicted molecular weight: 178 kDa).
|
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| IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/30.
|
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| Flow Cyt (Intra) |
1/50.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 210-250 kDa (predicted molecular weight: 178 kDa). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
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Flow Cyt (Intra)
1/50. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Found in the basement membranes (major component). -
Sequence similarities
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 3915 Human
- Entrez Gene: 226519 Mouse
- Entrez Gene: 117036 Rat
- Omim: 150290 Human
- SwissProt: P11047 Human
- SwissProt: Q5VYE7 Human
- SwissProt: Q6NVY8 Human
- SwissProt: P02468 Mouse
see all -
Alternative names
- LAMB2 antibody
- lamc1 antibody
- LAMC1_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in basement membrane of human pancreas (PMID: 15159456) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Flow Cytometry (Intracellular) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)](https://www.abcam.com/ps/products/233/ab233389/Images/ab233389-309873-anti-laminin-gamma-1-antibody-epr21199-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized A431 (human epidermoid carcinoma cell line) cell line labeling Laminin gamma 1 with ab233389 at 1/50 (red) compared with Rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)](https://www.abcam.com/ps/products/233/ab233389/Images/ab233389-309874-anti-laminin-gamma-1-antibody-epr21199-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)Laminin gamma 1 was immunoprecipitated from 0.35 mg of A431 (human epidermoid carcinoma cell line) whole cell lysate with ab233389 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233389 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: A431 whole lysate 10 μg (Input).
Lane 2: ab233389 IP in A431 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233389 in A431 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)](https://www.abcam.com/ps/products/233/ab233389/Images/ab233389-309872-anti-laminin-gamma-1-antibody-epr21199-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in basement membrane of rat stomach (PMID: 12571278) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)](https://www.abcam.com/ps/products/233/ab233389/Images/ab233389-309871-anti-laminin-gamma-1-antibody-epr21199-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in the basement membrane of mouse kidney (PMID: 21903675) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)](https://www.abcam.com/ps/products/233/ab233389/Images/ab233389-309869-anti-laminin-gamma-1-antibody-epr21199-western-blot.jpg)
Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)All lanes : Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/5000 dilution
Lane 1 : C6 (rat glial tumor glial cell line) whole cell lysate at 10 µg
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 3 : Rat heart lysate at 10 µg
Lane 4 : Rat kidney lysate at 10 µg
Lane 5 : Rat brain lysate at 10 µg
Lane 6 : Rat spleen lysate at 10 µg
Lane 7 : Mouse brain lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 178 kDa
Observed band size: 210-250 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 70 seconds. Lane 2: 15 seconds. Lanes 3 & 4: 10 seconds. Lanes 5 & 6: 3 minutes. Lane 7: 48 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 18263645, 11067943, 23857767, 28928891).
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![Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)](https://www.abcam.com/ps/products/233/ab233389/Images/ab233389-309868-anti-laminin-gamma-1-antibody-epr21199-western-blot.jpg)
Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)Lanes 1-3 & 6-7 : Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/5000 dilution
Lanes 4-5 : Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : NIH:OVCAR-3 (human ovary adenocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 6 : Human fetal heart lysate
Lane 7 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 6-7 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 178 kDa
Observed band size: 210-250 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1, 2 & 7: 15 seconds. Lane 3: 8 seconds. Lanes 4: 37 seconds. Lanes 5 & 6: 10 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 18263645, 11067943, 23857767, 28928891).
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![Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)](https://www.abcam.com/ps/products/233/ab233389/Images/ab233389-8-benefits-of-recombinant-antibodies.png)
Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab233389 has been referenced in 3 publications.
- Li Y et al. Proteomic Analysis of Uterine Tissues During Peri-Implantation Period in Mice with Experimentally Induced Adenomyosis that Treated with anti-Ngf: Implications for Cell-Cell Adhesion and Metabolic Processes. Reprod Sci 28:207-217 (2021). PubMed: 32676925
- You H et al. Sight and switch off: Nerve density visualization for interventions targeting nerves in prostate cancer. Sci Adv 6:eaax6040 (2020). PubMed: 32076639
- Balkawade RS et al. Podocyte-specific expression of Cre recombinase promotes glomerular basement membrane thickening. Am J Physiol Renal Physiol 316:F1026-F1040 (2019). PubMed: 30810063