Recombinant Anti-LAMP1 antibody [EPR21026] (ab208943)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21026] to LAMP1
- Suitable for: WB, IHC-P, IHC-Fr, IP, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-LAMP1 antibody [EPR21026]
See all LAMP1 primary antibodies -
Description
Rabbit monoclonal [EPR21026] to LAMP1 -
Host species
Rabbit -
Specificity
In our lab we observe staining in multiple tissues (spleen, lung, kidney etc.) in IHC-P with ab208943, but a lack of staining on mouse brain. We have received feedback from other researchers, that they also do not see staining in mouse brain with this antibody. Therefore we do not recommend using this reagent, for work on mouse brain, in IHC-P. For further information on this please contact our Technical Support team who will be happy to help.
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Tested applications
Suitable for: WB, IHC-P, IHC-Fr, IP, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lung, colon, kidney and liver lysates; Neuro-2a, RAW 264.7 and NIH/3T3 whole cell lysates. IHC-P: Mouse spleen and lung tissues. IHC-Fr: Mouse kidney tissue. ICC/IF: NIH/3T3 and Neuro-2a cells. Flow Cyt (intra): Neuro-2a cells. IP: NIH/3T3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21026 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 43 kDa).
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IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Works well in IHC-P on multiple tissues, but does not work on mouse brain, in this application. |
IHC-Fr |
1/100.
Perform heat mediated antigen retrieval using sodium citrate buffer (pH 6.0). |
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IP |
1/30.
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ICC/IF | (1) |
1/100.
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Flow Cyt (Intra) |
1/500.
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Notes |
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WB
1/1000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 43 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Works well in IHC-P on multiple tissues, but does not work on mouse brain, in this application. |
IHC-Fr
1/100. Perform heat mediated antigen retrieval using sodium citrate buffer (pH 6.0). |
IP
1/30. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/500. |
Target
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Function
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsO- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 16783 Mouse
- SwissProt: P11438 Mouse
- Unigene: 16716 Mouse
- Unigene: 475822 Mouse
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Alternative names
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
Images
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Immunofluorescent analysis of 100% methanol-fixed Neuro-2a (mouse neuroblastoma cell line) cells labeling LAMP1 with ab208943 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889), at 1/200 dilution (red).Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methonol-permeabilized Neuro-2a (mouse neuroblastoma cell line) cell line labeling LAMP1 with ab208943 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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All lanes : Anti-LAMP1 antibody [EPR21026] (ab208943) at 1/2000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 3 : Mouse kidney lysate at 20 µg
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 90-120 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1-2: 3 minutes; Lane 3: 5 seconds; Lane 4: 10 seconds.
Blocking and dilution buffer: 5% NFDM/TBST.
The varying band sizes are due to different levels of glycosylation (PMID: 10212251, PMID: 26246576).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling LAMP1 with ab208943 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on mouse spleen was observed, performed on a Leica Biosystems BOND® RX instrument (PMID: 22008915). Counter stained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.Note: BOND® is a registered trademark of Leica Biosystems Melbourne Pty Ltd.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling LAMP1 with ab208943 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line.The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889), at 1/200 dilution (red).Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling LAMP1 with ab208943 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on mouse spleen was observed, performed on a Leica Biosystems BOND® RX instrument (PMID: 22008915). Counter stained with hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.Note: BOND® is a registered trademark of Leica Biosystems Melbourne Pty Ltd.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse kidney tissue labeling LAMP1 with ab208943 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in the endothelial cells of glomeruli and epithelial cells of renal tubules (PMID: 23229015; PMID:23635510). The nuclear counter stain is DAPI (blue).Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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All lanes : Anti-LAMP1 antibody [EPR21026] (ab208943) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse colon lysate
Lane 3 : Mouse liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 90-120 kDa why is the actual band size different from the predicted?
Exposure time : Lanes 1-2: 15 seconds; Lane 3: 5 seconds.
Blocking and dilution buffer: 5% NFDM/TBST. -
LAMP1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab208943 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208943 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2: ab208943 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208943 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab208943 has been referenced in 8 publications.
- Fang S et al. Arsenic trioxide induces macrophage autophagy and atheroprotection by regulating ROS-dependent TFEB nuclear translocation and AKT/mTOR pathway. Cell Death Dis 12:88 (2021). PubMed: 33462182
- Xu H et al. Luteolin Attenuates Doxorubicin-Induced Cardiotoxicity Through Promoting Mitochondrial Autophagy. Front Physiol 11:113 (2020). PubMed: 32116805
- Xue J et al. Addition of High Molecular Weight Hyaluronic Acid to Fibroblast-Like Stromal Cells Modulates Endogenous Hyaluronic Acid Metabolism and Enhances Proteolytic Processing and Secretion of Versican. Cells 9:N/A (2020). PubMed: 32668663
- Chen H et al. Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production. JCI Insight 5:N/A (2020). PubMed: 32879135
- Sui B et al. A novel antiviral lncRNA, EDAL, shields a T309 O-GlcNAcylation site to promote EZH2 lysosomal degradation. Genome Biol 21:228 (2020). PubMed: 32873321
- Labadie T & Roy P A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion. PLoS Pathog 16:e1009015 (2020). PubMed: 33075107
- Serramito-Gómez I et al. Regulation of cytokine signaling through direct interaction between cytokine receptors and the ATG16L1 WD40 domain. Nat Commun 11:5919 (2020). PubMed: 33219218
- Trubetckaia O et al. Alpha-synuclein is strategically positioned for afferent modulation of midbrain dopamine neurons and is essential for cocaine preference. Commun Biol 2:418 (2019). PubMed: 31754648