Recombinant Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)](https://www.abcam.com/ps/products/247/ab247693/Images/ab247693-483279-ab108597121294antilamp1antibodyepr4204immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4204] to LAMP1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LAMP1 antibody [EPR4204] - BSA and Azide free
See all LAMP1 primary antibodies -
Description
Rabbit monoclonal [EPR4204] to LAMP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, JAR, A431 and Jurkat lysates. IHC-P: human liver carcinoma and kidney tissue.
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General notes
ab247693 is the carrier-free version of ab108597.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4204 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa).
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| Notes |
|---|
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IHC-P
1/100 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsO- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 3916 Human
- Omim: 153330 Human
- SwissProt: P11279 Human
- Unigene: 494419 Human
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Alternative names
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)
This data was developed using ab108597, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using unpurified ab108597 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)](https://www.abcam.com/ps/products/247/ab247693/Images/ab247693-483278-ab108597121295antilamp1antibodyepr4204westernblot.jpg)
Western blot - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)All lanes : Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597)
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDaThis data was developed using ab108597, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)](https://www.abcam.com/ps/products/247/ab247693/Images/ab247693-483277-ab108597304630antilamp1antibodyepr4204westernblot.jpg)
Western blot - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)All lanes : Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LAMP1 knockout HAP1 whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : Human Kidney whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 45 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?This data was developed using ab108597, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab108597 observed at 110 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108597 was shown to recognize LAMP1 in wild-type HAP1 cells as signal was lost at the expected MW in LAMP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and LAMP1 knockout samples were subjected to SDS-PAGE. ab108597 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)](https://www.abcam.com/ps/products/247/ab247693/Images/ab247693-483276-ab108597477522a.jpg)
Western blot - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)All lanes : Anti-LAMP1 antibody [EPR4204] - Lysosome Marker (ab108597) at 1/5000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate)
Predicted band size: 45 kDa
Exposure time: 5 secondsThis data was developed using ab108597, the same antibody clone in a different buffer formulation.
LAMP1 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 27061067, 15111122).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)](https://www.abcam.com/ps/products/247/ab247693/Images/ab247693-1-anti-lamp1-antibody-epr4204-immunohistochemistry-liver-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LAMP1 with purified ab108597 at 1/400 dilution (0.36 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108597).
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![Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)](https://www.abcam.com/ps/products/247/ab247693/Images/ab247693-2-benefits-of-recombinant-antibodies.png)
Anti-LAMP1 antibody [EPR4204] - BSA and Azide free (ab247693)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247693 has not yet been referenced specifically in any publications.
