Recombinant Anti-LAMP2 antibody [YCA-R24444-31 H2L1] - BSA and Azide free (Capture) (ab288606)
![Anti-LAMP2 antibody [YCA-R24444-31 H2L1] - BSA and Azide free (Capture) (ab288606)](https://www.abcam.com/ps/products/288/ab288606/Images/ab288606-1-benefitsofrecombinantantibodies.jpg "Anti-LAMP2 antibody [YCA-R24444-31 H2L1] - BSA and Azide free (Capture) (ab288606)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [YCA-R24444-31 H2L1] to LAMP2 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
Overview
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Product name
Anti-LAMP2 antibody [YCA-R24444-31 H2L1] - BSA and Azide free (Capture)
See all LAMP2 primary antibodies -
Description
Rabbit monoclonal [YCA-R24444-31 H2L1] to LAMP2 - BSA and Azide free (Capture) -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
General notes
ab288606 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human LAMP-2. The recommended pair for sandwich ELISA is:
Capture: ab288606, Human LAMP-2 Capture Antibody (unconjugated)
Detector: ab288624, Human LAMP-2 Detector Antibody (unconjugated)
The reference range value is 7.8125 - 2000 pg/mLOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
YCA-R24444-31 H2L1 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [YCA-R24444-155 H2L1] to LAMP2 - BSA and Azide free (Detector) (ab288624).
|
| Notes |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [YCA-R24444-155 H2L1] to LAMP2 - BSA and Azide free (Detector) (ab288624). |
Target
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Function
Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. -
Tissue specificity
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver. -
Involvement in disease
Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsO- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 3920 Human
- Omim: 309060 Human
- SwissProt: P13473 Human
- Unigene: 496684 Human
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Form
Alternative splicing produces 3 isoforms. -
Alternative names
- CD107 antigen like family member B antibody
- CD107 antigen-like family member B antibody
- CD107b antibody
see all
Images
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Anti-LAMP2 antibody [YCA-R24444-31 H2L1] - BSA and Azide free (Capture) (ab288606)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab288606 has not yet been referenced specifically in any publications.
