Recombinant Anti-LAMTOR2 antibody [EPR14378] (ab183514)
![Western blot - Anti-LAMTOR2 antibody [EPR14378] (ab183514)](https://www.abcam.com/ps/products/183/ab183514/Images/ab183514-389728-anti-lamtor2-antibody-epr14378-western-blot-human-lysate.jpg "Western blot - Anti-LAMTOR2 antibody [EPR14378] (ab183514)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14378] to LAMTOR2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LAMTOR2 antibody [EPR14378]
See all LAMTOR2 primary antibodies -
Description
Rabbit monoclonal [EPR14378] to LAMTOR2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, Raji and LnCaP cell lysates. IHC-P: Human thyroid tissue and Human gastric carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14378 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 14 kDa.
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| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000 - 1/2000. Predicted molecular weight: 14 kDa. |
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IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2. -
Involvement in disease
Defects in LAMTOR2 are the cause of immunodeficiency due to defect in MAPBP-interacting protein (ID-MAPBPIP) [MIM:610798]. This form of primary immunodeficiency syndrome includes congenital neutropenia, partial albinism, short stature and B-cell and cytotoxic T-cell deficiency. -
Sequence similarities
Belongs to the GAMAD family. -
Cellular localization
Late endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 28956 Human
- Entrez Gene: 83409 Mouse
- Entrez Gene: 295234 Rat
- Omim: 610389 Human
- SwissProt: Q9Y2Q5 Human
- SwissProt: Q9JHS3 Mouse
- Unigene: 632483 Human
- Unigene: 41680 Mouse
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Alternative names
- ENDAP antibody
- Endosomal adaptor protein p14 antibody
- HSPC003 antibody
see all
Images
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Western blot - Anti-LAMTOR2 antibody [EPR14378] (ab183514)All lanes : Anti-LAMTOR2 antibody [EPR14378] (ab183514) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LAMTOR2 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : LNCaP cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 14 kDaLanes 1-4: Merged signal (red and green). Green - ab183514 observed at 14 kDa. Red - loading control ab8245 observed at 37 kDa.
ab183514 Anti-LAMTOR2 antibody [EPR14378] was shown to specifically react with LAMTOR2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265828 (knockout cell lysate ab258020) was used. Wild-type and LAMTOR2 knockout samples were subjected to SDS-PAGE. ab183514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR2 antibody [EPR14378] (ab183514)](https://www.abcam.com/ps/products/183/ab183514/Images/ab183514-215188-ab183514-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR2 antibody [EPR14378] (ab183514)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma labeling LAMTOR2 using ab183514 at a 1/50 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR2 antibody [EPR14378] (ab183514)](https://www.abcam.com/ps/products/183/ab183514/Images/ab183514-215185-ab183514-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR2 antibody [EPR14378] (ab183514)Immunohistochemical analysis of paraffin-embedded Human thyroid tissue labeling LAMTOR2 using ab183514 at a 1/50 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-LAMTOR2 antibody [EPR14378] (ab183514)](https://www.abcam.com/ps/products/183/ab183514/Images/ab183514-215184-ab183514.jpg)
Western blot - Anti-LAMTOR2 antibody [EPR14378] (ab183514)All lanes : Anti-LAMTOR2 antibody [EPR14378] (ab183514) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : LnCaP cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa -
![Anti-LAMTOR2 antibody [EPR14378] (ab183514)](https://www.abcam.com/ps/products/183/ab183514/Images/ab183514-5-benefits-of-recombinant-antibodies.png)
Anti-LAMTOR2 antibody [EPR14378] (ab183514)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab183514 has not yet been referenced specifically in any publications.