Anti-Lens Fiber Cell Marker antibody [ZL-1] (ab185979)
![Immunohistochemistry (Frozen sections) - Anti-Lens Fiber Cell Marker antibody [ZL-1] (ab185979)](https://www.abcam.com/ps/products/185/ab185979/Images/ab185979-224055-anti-lens-fiber-cell-marker-antibody-zl-1-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Lens Fiber Cell Marker antibody [ZL-1] (ab185979)")
Key features and details
- Mouse monoclonal [ZL-1] to Lens Fiber Cell Marker
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-Lens Fiber Cell Marker antibody [ZL-1] -
Description
Mouse monoclonal [ZL-1] to Lens Fiber Cell Marker -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Zebrafish
Does not react with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
SDS-PAGE -
Purification notes
The purity of ab185979 is near homogeneity, as judged by SDS-PAGE. ab185979 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. -
Clonality
Monoclonal -
Clone number
ZL-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
Target
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Relevance
The lens fibers form the bulk of the lens. They are long, thin, transparent cells, firmly packed, with diameters typically between 4-7 micrometres and lengths of up to 12 mm long. These tightly packed layers of lens fibers are referred to as laminae. The lens fibers are linked together via gap junctions and interdigitations of the cells that resemble "ball and socket" forms. The lens is split into regions depending on the age of the lens fibers of a particular layer. Moving outwards from the central, oldest layer, the lens is split into an embryonic nucleus, the fetal nucleus, the adult nucleus, and the outer cortex. New lens fibers, generated from the lens epithelium, are added to the outer cortex. Mature lens fibers have no organelles or nuclei -
Alternative names
- Lens fiber cell Marker antibody
- zl1 antibody
Images
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Immunohistochemistry (Frozen sections) - Anti-Lens Fiber Cell Marker antibody [ZL-1] (ab185979)This image is courtesy of an Abreview submitted by Dr Ryan MacDonald
ab185979 staining the lens in 4 dpf zebrafish embryo retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and permeabilization was performed using 0.5% Triton X. Blocking of the sample was done with 5% BSA in PBS containing 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab185979 at 1/100 in blocking solution for 16h at 4°C. An alexa 647 conjugated goat anti-mouse polyclonal antibody at 1/500 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The lens can be observed.
Datasheets and documents
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Datasheet download
References (1)
ab185979 has been referenced in 1 publication.
- Hao Y et al. Interplay of MPP5a with Rab11 synergistically builds epithelial apical polarity and zonula adherens. Development 147:N/A (2020). PubMed: 33060129
