Recombinant Anti-LGR5 antibody [EPR3065Y] (ab75850)
![Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)](https://www.abcam.com/ps/products/75/ab75850/Images/ab75850-420472-anti-lgr5-antibody-epr3065y-westernblot-skeletal-muscle-human.jpg "Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3065Y] to LGR5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-LGR5 antibody [EPR3065Y]
See all LGR5 primary antibodies -
Description
Rabbit monoclonal [EPR3065Y] to LGR5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Fetal skeletal, human and mouse skeletal, mouse and rat brain lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3065Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 100 kDa.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 100 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
-
Function
Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling. -
Tissue specificity
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 8549 Human
- Entrez Gene: 14160 Mouse
- Entrez Gene: 299802 Rat
- Omim: 606667 Human
- SwissProt: O75473 Human
- SwissProt: Q9Z1P4 Mouse
- Unigene: 658889 Human
- Unigene: 42103 Mouse
-
Alternative names
- FEX antibody
- G protein coupled receptor 49 antibody
- G protein coupled receptor 67 antibody
see all
Images
-
Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (Purified) + Human skeletal muscle lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDaBlocking Buffer and concentration: 5% NFDM/TBST
-
![Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)](https://www.abcam.com/ps/products/75/ab75850/Images/ab75850-420471-anti-lgr5-antibody-epr3065y-westernblot-mouse-rat.jpg)
Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)All lanes : Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (Purified)
Lane 1 : Mouse skeletal muscle lysate
Lane 2 : Rat skeletal muscle lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.We are unsure about the identity of the additional band.
Blocking Buffer and concentration: 5% NFDM/TBST
-
![Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)](https://www.abcam.com/ps/products/75/ab75850/Images/ab75850-90973-anti-lgr5-antibody-epr3065y-western-blot.jpg)
Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (unpurified) + Fetal skeletal muscle tissue lysate at 10 µg
Secondary
HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa -
![Anti-LGR5 antibody [EPR3065Y] (ab75850)](https://www.abcam.com/ps/products/75/ab75850/Images/ab75850-6-benefits-of-recombinant-antibodies.png)
Anti-LGR5 antibody [EPR3065Y] (ab75850)
Datasheets and documents
-
SDS download
-
Datasheet download
References (52)
ab75850 has been referenced in 52 publications.
- Chiang HY et al. IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence. Nat Commun 13:874 (2022). PubMed: 35169117
- Chang ZY et al. Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35269806
- Choi KM et al. The interferon-inducible protein viperin controls cancer metabolic reprogramming to enhance cancer progression. J Clin Invest 132:N/A (2022). PubMed: 36227691
- Chamseddine D et al. The mitochondrial UPR regulator ATF5 promotes intestinal barrier function via control of the satiety response. Cell Rep 41:111789 (2022). PubMed: 36516750
- Dunbar K et al. Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1. Cell Mol Gastroenterol Hepatol 11:465-489 (2021). PubMed: 32971322