Anti-LGR5 antibody [OTI2A2] (ab273092)
![Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (ab273092)](https://www.abcam.com/ps/products/273/ab273092/Images/ab273092-390949-anti-lrg5-antibody-flow-cytometry-hek239-cells.JPG "Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (ab273092)")
Key features and details
- Mouse monoclonal [OTI2A2] to LGR5
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-LGR5 antibody [OTI2A2]
See all LGR5 primary antibodies -
Description
Mouse monoclonal [OTI2A2] to LGR5 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human LGR5 aa 250-550. Genbank: NP_003658)
Database link: O75473 -
Positive control
- Flow Cyt: HEK-293T cells transfected with pCMV6-ENTRY LGR5 cDNA ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY LGR5. HT-29 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI2A2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/100.
|
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| ICC/IF |
1/100.
|
| Notes |
|---|
|
Flow Cyt
1/100. |
|
ICC/IF
1/100. |
Target
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Function
Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling. -
Tissue specificity
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8549 Human
- Omim: 606667 Human
- SwissProt: O75473 Human
- Unigene: 658889 Human
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Alternative names
- FEX antibody
- G protein coupled receptor 49 antibody
- G protein coupled receptor 67 antibody
see all
Images
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Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (ab273092)
Flow cytometric analysis of HEK-293T cells transfected with either LGR5 overexpressed plasmid (Red) or empty vector control plasmid (Blue). Cells were immunostained with ab273092 at 1/100 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)](https://www.abcam.com/ps/products/273/ab273092/Images/ab273092-390950-anti-lrg5-antibody-immuocytochemistry-ht29-cells.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)Immunofluorescent staining of HT-29 cells labeling LGR5 using ab273092 at 1/100 dilution (green). Actin filaments were labeled with TRITC-phalloidin (red). The nuclear counter stain is DAPI (blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)](https://www.abcam.com/ps/products/273/ab273092/Images/ab273092-390953-anti-lrg5-antibody-immuocytochemistry-cos7cells.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)Immunofluorescent staining of COS-7 cells transiently transfected by pCMV6-ENTRY LGR5, labeling LGR5 using ab273092 at 1/100 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab273092 has not yet been referenced specifically in any publications.