Name: Anti-Lhx2/LH2 antibody [EPR20449]
Brand: RabMAb
SKU: ab184337
Rating: 5 (5 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20449] to Lhx2/LH2
Suitable for: WB, IHC-Fr, IP, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-Lhx2/LH2 antibody [EPR20449]
See all Lhx2/LH2 primary antibodies
Description

Rabbit monoclonal [EPR20449] to Lhx2/LH2
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Fr, IP, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged rat Lhx2/LH2 recombinant protein fragment (aa43-426); E18 rat brain lysate; E12.5 mouse brain lysate. IHC-P: Mouse olfactory epithelium, Nose skin of P0 mouse, Mouse E14.5, Rat olfactory epithelium, Nose skin of P0 rat and rat E14.5 tissues. IHC-Fr: Mouse E14.5 and rat E14.5 tissues. IP: E18 rat brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20449
Isotype

IgG
Research areas

Alternative Versions
- Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 44 kDa).
IHC-Fr	(2)	1/500. Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
IP		1/30.
IHC-P	(1)	1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 44 kDa).
IHC-Fr 1/500. Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
IP 1/30.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.
Sequence similarities

Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
Domain

LIM domains are necessary for transcription activation.
Cellular localization

Nucleus.
Target information above from: UniProt accession P50458 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 9355 Human
- Entrez Gene: 16870 Mouse
- Entrez Gene: 296706 Rat
- Omim: 603759 Human
- SwissProt: P50458 Human
- SwissProt: Q9Z0S2 Mouse
- SwissProt: P36198 Rat
- Unigene: 696425 Human
- Unigene: 142856 Mouse
- Unigene: 81063 Rat
see all
Alternative names
- hLhx2 antibody
- Homeobox protein LH-2 antibody
- Homeobox protein LH2 antibody
- LH2 antibody
- LHX2 antibody
- LHX2_HUMAN antibody
- LIM homeobox 2 antibody
- LIM homeobox protein 2 antibody
- LIM HOX gene 2 antibody
- LIM/homeobox protein Lhx2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded mouse olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse olfactory epithelium [PMID: 15456728].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on vomeronasal organ of mouse E14.5 [PMID: 27521061].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

All lanes : Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution

Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : 293T transfected with human Lhx2/LH2 (WT) expression vector containing a GFP-tag, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking buffer and concentration : 5% NFDM/TBST

Diluting buffer and concentration : 5% NFDM/TBST
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

All lanes : Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution

Lane 1 : His-tagged rat Lhx2/LH2 recombinant protein fragment (aa43-426)
Lane 2 : His-tagged rat Lhx9 recombinant protein fragment (aa54-172)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 44 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Lhx2/LH2 was immunoprecipitated from 0.35 mg of E18 rat brain lysate with ab184337 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184337 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: E18 rat brain lysate, 10 μg (Input).

Lane 2: ab184337 IP in E18 rat brain lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184337 in E18 rat brain lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded Mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of mouse E14.5 [PMID: 25071464].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded rat olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat olfactory epithelium [PMID: 15456728].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on olfactory epithelium of rat E14.5 [PMID: 15456728].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution + E18 rat brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/200 dilution + E12.5 mouse brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Molecular weight observed is consistent with what has been described in the literature, PMID 22028024, PMID 25128627.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded Rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of rat E14.5 [PMID: 25071464].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded nose skin of P0 mouse labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of mouse nose skin [PMID: 20386748].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunohistochemical analysis of paraffin-embedded nose skin of P0 rat labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of rat nose skin [PMID: 20386748].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab184337? Please let us know so that we can cite the reference in this datasheet.

ab184337 has been referenced in 4 publications.

Dobie R et al. Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis. Cell Rep 29:1832-1847.e8 (2019). PubMed: 31722201
Song H et al. LHX2 promotes malignancy and inhibits autophagy via mTOR in osteosarcoma and is negatively regulated by miR-129-5p. Aging (Albany NY) 11:9794-9810 (2019). PubMed: 31724536
Han X et al. FoxG1 Directly Represses Dentate Granule Cell Fate During Forebrain Development. Front Cell Neurosci 12:452 (2018). PubMed: 30532694
Liu B et al. Forced Expression of Foxg1 in the Cortical Hem Leads to the Transformation of Cajal-Retzius Cells into Dentate Granule Neurons. J Dev Biol 6:N/A (2018). PubMed: 29949945

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Chicken Tissue sections (Chicken spinal cord embryo 5.5days)

Permeabilization

Yes - Triton X100 0.1%

Specification

Chicken spinal cord embryo 5.5days

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Gerard Elberg

Verified customer

Submitted Jun 02 2021

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Human embryonic spinal cord)

Permeabilization

Specification

Human embryonic spinal cord

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Feb 25 2021

Application

IHC - Wholemount

Sample

Human Embryo (Human embryonic Spinal cord)

Specification

Human embryonic Spinal cord

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Feb 25 2021

Application

IHC - Wholemount

Sample

Mouse Embryo (E12.5 Mouse embryo)

Specification

E12.5 Mouse embryo

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Feb 25 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: ph9

Permeabilization

Specification

Brain

Blocking step

Protain block, serum free(dako) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 09 2020

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (4)

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-Lhx2/LH2 antibody [EPR20449]

Immunohistochemistry (Frozen sections) abreview for Anti-Lhx2/LH2 antibody [EPR20449]

IHC - Wholemount abreview for Anti-Lhx2/LH2 antibody [EPR20449]

IHC - Wholemount abreview for Anti-Lhx2/LH2 antibody [EPR20449]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lhx2/LH2 antibody [EPR20449]