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RecombinantRabMAb

Recombinant Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
  • Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20449] to Lhx2/LH2
  • Suitable for: WB, IHC-Fr, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)

View more associated products

Overview

  • Product name

    Anti-Lhx2/LH2 antibody [EPR20449]
    See all Lhx2/LH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20449] to Lhx2/LH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged rat Lhx2/LH2 recombinant protein fragment (aa43-426); E18 rat brain lysate; E12.5 mouse brain lysate. IHC-P: Mouse olfactory epithelium, Nose skin of P0 mouse, Mouse E14.5, Rat olfactory epithelium, Nose skin of P0 rat and rat E14.5 tissues. IHC-Fr: Mouse E14.5 and rat E14.5 tissues. IP: E18 rat brain lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20449
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • HOX
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • LIM
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules

Associated products

  • Alternative Versions

    • Anti-Lhx2/LH2 antibody [EPR20449] - BSA and Azide free (ab236037)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 44 kDa).
IHC-Fr (2)
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

IP
1/30.
IHC-P (1)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 44 kDa).
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

IP
1/30.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Domain

    LIM domains are necessary for transcription activation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P50458 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9355 Human
    • Entrez Gene: 16870 Mouse
    • Entrez Gene: 296706 Rat
    • Omim: 603759 Human
    • SwissProt: P50458 Human
    • SwissProt: Q9Z0S2 Mouse
    • SwissProt: P36198 Rat
    • Unigene: 696425 Human
    • Unigene: 142856 Mouse
    • Unigene: 81063 Rat
    see all
  • Alternative names

    • hLhx2 antibody
    • Homeobox protein LH-2 antibody
    • Homeobox protein LH2 antibody
    • LH2 antibody
    • LHX2 antibody
    • LHX2_HUMAN antibody
    • LIM homeobox 2 antibody
    • LIM homeobox protein 2 antibody
    • LIM HOX gene 2 antibody
    • LIM/homeobox protein Lhx2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

    Immunohistochemical analysis of paraffin-embedded mouse olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse olfactory epithelium [PMID: 15456728].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on vomeronasal organ of mouse E14.5 [PMID: 27521061].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    All lanes : Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : 293T transfected with human Lhx2/LH2 (WT) expression vector containing a GFP-tag, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking buffer and concentration : 5% NFDM/TBST

    Diluting buffer and concentration : 5% NFDM/TBST

     

  • Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    All lanes : Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution

    Lane 1 : His-tagged rat Lhx2/LH2 recombinant protein fragment (aa43-426)
    Lane 2 : His-tagged rat Lhx9 recombinant protein fragment (aa54-172)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Immunoprecipitation - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

    Lhx2/LH2 was immunoprecipitated from 0.35 mg of E18 rat brain lysate with ab184337 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184337 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: E18 rat brain lysate, 10 μg (Input).

    Lane 2: ab184337 IP in E18 rat brain lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184337 in E18 rat brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

    Immunohistochemical analysis of paraffin-embedded Mouse E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of mouse E14.5 [PMID: 25071464].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

    Immunohistochemical analysis of paraffin-embedded rat olfactory epithelium tissue labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat olfactory epithelium [PMID: 15456728].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Immunohistochemistry (Frozen sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on olfactory epithelium of rat E14.5 [PMID: 15456728].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/1000 dilution + E18 rat brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Western blot - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Anti-Lhx2/LH2 antibody [EPR20449] (ab184337) at 1/200 dilution + E12.5 mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Molecular weight observed is consistent with what has been described in the literature, PMID 22028024, PMID 25128627.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

    Immunohistochemical analysis of paraffin-embedded Rat E14.5 labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on telencephalon and olfactory epithelium of rat E14.5 [PMID: 25071464].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

    Immunohistochemical analysis of paraffin-embedded nose skin of P0 mouse labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of mouse nose skin [PMID: 20386748].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

    Immunohistochemical analysis of paraffin-embedded nose skin of P0 rat labeling Lhx2/LH2 with ab184337 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on hair follicle of rat nose skin [PMID: 20386748].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)
    Anti-Lhx2/LH2 antibody [EPR20449] (ab184337)

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (4)

Publishing research using ab184337? Please let us know so that we can cite the reference in this datasheet.

ab184337 has been referenced in 4 publications.

  • Dobie R  et al. Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis. Cell Rep 29:1832-1847.e8 (2019). PubMed: 31722201
  • Song H  et al. LHX2 promotes malignancy and inhibits autophagy via mTOR in osteosarcoma and is negatively regulated by miR-129-5p. Aging (Albany NY) 11:9794-9810 (2019). PubMed: 31724536
  • Han X  et al. FoxG1 Directly Represses Dentate Granule Cell Fate During Forebrain Development. Front Cell Neurosci 12:452 (2018). PubMed: 30532694
  • Liu B  et al. Forced Expression of Foxg1 in the Cortical Hem Leads to the Transformation of Cajal-Retzius Cells into Dentate Granule Neurons. J Dev Biol 6:N/A (2018). PubMed: 29949945

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Lhx2/LH2 antibody [EPR20449]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Chicken spinal cord embryo 5.5days)
Permeabilization
Yes - Triton X100 0.1%
Specification
Chicken spinal cord embryo 5.5days
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Gerard Elberg

Verified customer

Submitted Jun 02 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Lhx2/LH2 antibody [EPR20449]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human embryonic spinal cord)
Permeabilization
No
Specification
Human embryonic spinal cord
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Feb 25 2021

IHC - Wholemount abreview for Anti-Lhx2/LH2 antibody [EPR20449]

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Human Embryo (Human embryonic Spinal cord)
Specification
Human embryonic Spinal cord
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Feb 25 2021

IHC - Wholemount abreview for Anti-Lhx2/LH2 antibody [EPR20449]

Good
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 Mouse embryo)
Specification
E12.5 Mouse embryo
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Feb 25 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lhx2/LH2 antibody [EPR20449]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ph9
Permeabilization
No
Specification
Brain
Blocking step
Protain block, serum free(dako) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 09 2020

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