Recombinant Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697)
![Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697)](https://www.abcam.com/ps/products/124/ab124697/Images/ab124697-138480-anti-lhx3-lim-antibody-epr33442-western-blot.jpg "Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3344(2)] to LHX3/LIM
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-LHX3/LIM antibody [EPR3344(2)]
See all LHX3/LIM primary antibodies -
Description
Rabbit monoclonal [EPR3344(2)] to LHX3/LIM -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide within Human LHX3/LIM aa 300-400. The exact sequence is proprietary.
-
Positive control
- 293T and Jurkat whole cell lysate (ab7899), Human brain and fetal lung tissue lysates; permeabilized Jurkat cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.84 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3344(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
Application notes
Is unsuitable for ICC/IF or IHC-P.
Target
-
Function
Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1. -
Involvement in disease
Defects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
Domain
The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 8022 Human
- Entrez Gene: 16871 Mouse
- Entrez Gene: 170671 Rat
- Omim: 600577 Human
- SwissProt: Q9UBR4 Human
- SwissProt: P50481 Mouse
- Unigene: 148427 Human
- Unigene: 386765 Mouse
-
Alternative names
- CPHD 3 antibody
- CPHD3 antibody
- DKFZp762A2013 antibody
see all
Images
-
Western blot - Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697)All lanes : Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697) at 1/1000 dilution
Lane 1 : 293T(Human embryonic kidney epithelial cell) cell lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 43 kDa -
![Flow Cytometry (Intracellular) - Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697)](https://www.abcam.com/ps/products/124/ab124697/Images/ab124697-138479-anti-lhx3-lim-antibody-epr33442-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697)ab124697 at 1/10 dilution, staining LHX3/LIM in permeabilized Jurkat cells by intracellular flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
-
![OI-RD Scanning - Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697)](https://www.abcam.com/ps/products/124/ab124697/Images/ab124697-138478-anti-lhx3-lim-antibody-epr33442.jpg)
OI-RD Scanning - Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697) -
![Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697)](https://www.abcam.com/ps/products/124/ab124697/Images/ab124697-4-benefits-of-recombinant-antibodies.png)
Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697)
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab124697 has been referenced in 2 publications.
- Feng Y et al. HOXD8/DIAPH2-AS1 epigenetically regulates PAX3 and impairs HTR-8/SVneo cell function under hypoxia. Biosci Rep 39:N/A (2019). PubMed: 30626726
- Patani R et al. Retinoid-independent motor neurogenesis from human embryonic stem cells reveals a medial columnar ground state. Nat Commun 2:214 (2011). PubMed: 21364553