Recombinant Anti-LHX6 antibody [EPR26352-101] (ab300441)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHX6 antibody [EPR26352-101] (AB300441)](https://www.abcam.com/ps/products/300/ab300441/Images/AB300441-487063-anti-LHX6-antibody-EPR26352101-IHC-mouse-brain.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHX6 antibody [EPR26352-101] (AB300441)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26352-101] to LHX6
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-LHX6 antibody [EPR26352-101]
See all LHX6 primary antibodies -
Description
Rabbit monoclonal [EPR26352-101] to LHX6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-Fr,IP or WB -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse embryonic cerebrum E14.5, Mouse cerebrum, Rat cerebrum FFPE tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26352-101 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Probable transcription factor required for the expression of a subset of genes involved in interneurons migration and development. Functions in the specification of cortical interneuron subtypes and in the migration of GABAergic interneuron precursors from the subpallium to the cerebral cortex. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 16874 Mouse
- Entrez Gene: 311901 Rat
- SwissProt: O88706 Mouse
- SwissProt: Q6P1H2 Mouse
- SwissProt: Q9R1R0 Mouse
- Unigene: 12881 Mouse
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Alternative names
- LHX 6 antibody
- LHX 6.1 antibody
- LHX6 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHX6 antibody [EPR26352-101] (AB300441)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum labeling LHX6 with ab300441 at 1/500 dilution (0.942 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on mouse cerebrum. The section was incubated with ab300441 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHX6 antibody [EPR26352-101] (AB300441)](https://www.abcam.com/ps/products/300/ab300441/Images/AB300441-487064-anti-LHX6-antibody-EPR26352101-IHC-mouse-fetus-brain.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHX6 antibody [EPR26352-101] (AB300441)Immunohistochemical analysis of paraffin-embedded mouse embryonic cerebrum E14.5 labeling LHX6 with ab300441 at 1/500 dilution (0.942 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on mouse embryonic cerebrum E14.5 (PMID: 33479483). The section was incubated with ab300441 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHX6 antibody [EPR26352-101] (AB300441)](https://www.abcam.com/ps/products/300/ab300441/Images/AB300441-487065-anti-LHX6-antibody-EPR26352101-IHC-rat-brain.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LHX6 antibody [EPR26352-101] (AB300441)Immunohistochemical analysis of paraffin-embedded rat cerebrum labeling LHX6 with ab300441 at 1/500 dilution (0.942 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Nuclear staining on rat cerebrum. The section was incubated with ab300441 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Anti-LHX6 antibody [EPR26352-101] (AB300441)](https://www.abcam.com/ps/products/300/ab300441/Images/AB300441-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-LHX6 antibody [EPR26352-101] (AB300441)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300441 has not yet been referenced specifically in any publications.