Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)
![Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)](https://www.abcam.com/ps/products/119/ab119084/Images/LIM-Kinase-1-Primary-antibodies-ab119084-1.jpg "Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)")
Key features and details
- Mouse monoclonal [OTI3G3] to LIM Kinase 1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-LIM Kinase 1 antibody [OTI3G3]
See all LIM Kinase 1 primary antibodies -
Description
Mouse monoclonal [OTI3G3] to LIM Kinase 1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human LIM Kinase 1. Produced in HEK-293T cells. NP_002305.
Database link: P53667 -
Positive control
- WB: HEK-293T cells transfected with LIM Kinase 1. ICC/IF: COS-7 cells transiently transfected with LIM Kinase 1. IHC-P: Human endometrium adenocarcinoma and lymphoma tissue.
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General notes
The clone number has been updated from 3G3 to OTI3G3, both clone numbers name the same clone.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI3G3 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 73 kDa.
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| ICC/IF |
1/100.
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| IHC-P |
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/2000. Predicted molecular weight: 73 kDa. |
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ICC/IF
1/100. |
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IHC-P
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. -
Tissue specificity
Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. -
Involvement in disease
Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. -
Cellular localization
Cytoplasm. Cell projection > growth cone. - Information by UniProt
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Database links
- Entrez Gene: 3984 Human
- Omim: 601329 Human
- SwissProt: P53667 Human
- Unigene: 647035 Human
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Alternative names
- EC 2.7.1.37 antibody
- LIM domain containing protein kinase antibody
- LIM domain kinase 1 antibody
see all
Images
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Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)All lanes : Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected the pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected the pCMV6-ENTRY LIM Kinase 1
Lysates/proteins at 5 µg per lane.
Predicted band size: 73 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)](https://www.abcam.com/ps/products/119/ab119084/Images/LIM-Kinase-1-Primary-antibodies-ab119084-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)Immunofluorescent staining of COS-7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1 using ab119084 at a dilution of 1/100.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)](https://www.abcam.com/ps/products/119/ab119084/Images/ab119084-314181-ab119084ihc2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human endometrium adenocarcinoma tissue using ab119084 at a 1/150 dilution. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)](https://www.abcam.com/ps/products/119/ab119084/Images/ab119084-314179-ab119084ihc1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lymphoma tissue using ab119084 at a 1/150 dilution. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab119084 has been referenced in 3 publications.
- Ravindran S et al. BDNF Induced Translation of Limk1 in Developing Neurons Regulates Dendrite Growth by Fine-Tuning Cofilin1 Activity. Front Mol Neurosci 12:64 (2019). PubMed: 30949027
- Yao J et al. miR-133b regulates proliferation and apoptosis in high-glucose-induced human retinal endothelial cells by targeting ras homolog family member A. Int J Mol Med 42:839-850 (2018). PubMed: 29786744
- Yu L et al. c-Maf inducing protein inhibits cofilin-1 activity and alters podocyte cytoskeleton organization. Mol Med Rep 16:4955-4963 (2017). PubMed: 28791377