Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)
Key features and details
- Mouse monoclonal [5G5] to Lipocalin-2 / NGAL
- Suitable for: WB, IHC-FoFr, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Lipocalin-2 / NGAL antibody [5G5]
See all Lipocalin-2 / NGAL primary antibodies -
Description
Mouse monoclonal [5G5] to Lipocalin-2 / NGAL -
Host species
Mouse -
Specificity
ab23477 is specific for Lipocalin-2 / NGAL, native and denatured forms. -
Tested applications
Suitable for: WB, IHC-FoFr, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Lipocalin-2/ NGAL. Full length native human neutrophil gelatinase associated lipocalin isolated from neutrophils.
-
Positive control
- WB: SW480 cell lysate. IHC-P: PaCa, chronic pancreatitis and normal pancreas tissue sections.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at 4°C or -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5G5 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000. Predicted molecular weight: 22 kDa.
|
IHC-FoFr |
Use at an assay dependent concentration.
|
|
IHC-P | (1) |
Use at an assay dependent concentration.
|
Notes |
---|
WB
1/1000. Predicted molecular weight: 22 kDa. |
IHC-FoFr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Target
-
Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
-
Database links
- Entrez Gene: 3934 Human
- Omim: 600181 Human
- SwissProt: P80188 Human
- Unigene: 204238 Human
-
Alternative names
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
see all
Images
-
All lanes : Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477) at 1/1000 dilution
Lane 1 : Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : LCN2 knockout SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab23477 observed at 23 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.
ab23477 was shown to react with Lipocalin-2 / NGAL in wild-type SW480 cells in Western blot with loss of signal observed in LCN2 knockout knockout cell line ab270486 (knockout cell lysate ab270509). Wild-type SW480 and LCN2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab23477 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)This image is courtesy of an anonymous Abreview
ab23477 staining human skin tissue sections by IHC-P. Sections were fixed in 10% neutral buffered formalin and subjected to heat mediated antigen retrieval prior to blocking with 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 20 hours at 4°C. A biotinylated horse anti-mouse antibody diluted 1/200 was used as the secondary.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human PaCa tissue sections labelling Lipocalin 2 with ab23477.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human chronic pancreatits tissue sections labelling Lipocalin-2 / NGAL with ab23477.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [5G5] (ab23477)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human pancreas tissue sections labelling Lipocalin-2 / NGAL with ab23477.
Datasheets and documents
-
SDS download
-
Datasheet download
References (15)
ab23477 has been referenced in 15 publications.
- Cione E et al. Expression of MMP-2, MMP-9, and NGAL in Tissue and Serum of Patients with Vascular Aneurysms and Their Modulation by Statin Treatment: A Pilot Study. Biomolecules 10:N/A (2020). PubMed: 32111073
- Guo Y et al. NGAL protects in nasopharyngeal carcinoma by inducing apoptosis and blocking epithelial-mesenchymal transition. Oncol Lett 19:3711-3718 (2020). PubMed: 32391093
- Monisha J et al. NGAL is Downregulated in Oral Squamous Cell Carcinoma and Leads to Increased Survival, Proliferation, Migration and Chemoresistance. Cancers (Basel) 10:N/A (2018). PubMed: 29996471
- Ku AT et al. TCF7L1 promotes skin tumorigenesis independently of ß-catenin through induction of LCN2. Elife 6:N/A (2017). PubMed: 28467300
- Wang X et al. iTRAQ-Based Proteomics Screen identifies LIPOCALIN-2 (LCN-2) as a potential biomarker for colonic lateral-spreading tumors. Sci Rep 6:28600 (2016). WB, IHC . PubMed: 27339395