Recombinant Anti-Liver Arginase antibody [EPR6672(B)] (ab133543)

False colour image of Western blot: Anti-Liver Arginase antibody [EPR6672(B)] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133543 was shown to bind specifically to Liver Arginase. A band was observed at 36 kDa in wild-type HepG2 cell lysates with no signal observed at this size in arg1 knockout cell line ab281603 (knockout cell lysate ab282955). To generate this image, wild-type and arg1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

Blocking and diluting buffer: 5% NFDM/TBST.

ab133543 staining liver arginase in paraffin embedded human hepatocellular cancer tissue sections by Immunohistochemistry.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Samples were incubated with primary antibody at 1:2000 dilution (0.13 μg/ml).

A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody.

Hematoxylin was used as a counterstain.

Cytoplasmic and nuclear staining on human hepatocellular cancer.

Lane 1 (input): Human fetal liver whole cell lysate, 10μg
Lane 2: Human fetal liver whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133543 in Human fetal liver whole cell lysate

Ab133543 immunoprecipitating liver arginase in Human fetal liver whole cell lysates. Primary antibody was used at a 1:1000 dilution (0.27 μg/ml). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:5000 dilution.  Capture antibody was used at 1:20 dilution (1.3μg in 0.35mg lysates).

Blocking and diluting buffer used: 5% NFDM/TBST.

Tissue Microarrays stained for "Anti-Liver Arginase antibody [EPR6672(B)]” using "ab133543" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab133543 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.