Anti-LRP4 antibody [N207/27] - N-terminal (ab174637)
![Immunocytochemistry/ Immunofluorescence - Anti-LRP4 antibody [N207/27] - N-terminal (ab174637)](https://www.abcam.com/ps/products/174/ab174637/Images/ab174637-311529-anti-lrp4-antibody-n207-27-n-terminal-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LRP4 antibody [N207/27] - N-terminal (ab174637)")
Key features and details
- Mouse monoclonal [N207/27] to LRP4 - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-LRP4 antibody [N207/27] - N-terminal
See all LRP4 primary antibodies -
Description
Mouse monoclonal [N207/27] to LRP4 - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment (His-tag) within Mouse LRP4 aa 1-350 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Extracellular domain.
Database link: Q8VI56 -
Epitope
extracellular domain -
Positive control
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S207-27 to N207/27, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N207/27 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
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| WB |
1/1000. Predicted molecular weight: 212 kDa.
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| Notes |
|---|
|
ICC/IF
1/100. |
|
WB
1/1000. Predicted molecular weight: 212 kDa. |
Target
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Function
Potential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes. Involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. -
Tissue specificity
Expressed in several regions of the brain. -
Involvement in disease
Defects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS) [MIM:212780]. It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs. -
Sequence similarities
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 8 LDL-receptor class A domains.
Contains 20 LDL-receptor class B repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4038 Human
- Entrez Gene: 228357 Mouse
- Entrez Gene: 83469 Rat
- Omim: 604270 Human
- SwissProt: O75096 Human
- SwissProt: Q8VI56 Mouse
- SwissProt: Q9Z319 Mouse
- SwissProt: Q9QYP1 Rat
see all -
Alternative names
- KIAA0816 antibody
- LDLR dan antibody
- Low density lipoprotein receptor related protein 4 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LRP4 antibody [N207/27] - N-terminal (ab174637)
4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for LRP4 (green) using ab174637 at 1/100 dilution in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1:100. Actin is detected with Phalloidin Texas Red F-Actin stain (red). The nuclear counterstain is DAPI (blue).
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![Western blot - Anti-LRP4 antibody [N207/27] - N-terminal (ab174637)](https://www.abcam.com/ps/products/174/ab174637/Images/ab174637-311528-anti-lrp4-antibody-n207-27-n-terminal-western-blot.jpg)
Western blot - Anti-LRP4 antibody [N207/27] - N-terminal (ab174637)Lane 1 : Anti-LRP4 antibody [N207/27] - N-terminal (ab174637) at 1/500 dilution
Lane 2 : Anti-LRP4 antibody [N207/27] - N-terminal (ab174637) at 1/1000 dilution
Lane 3 : Anti-LRP4 antibody [N207/27] - N-terminal (ab174637) at 1/2000 dilution
All lanes : Rat brain membrane lysate
Predicted band size: 212 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab174637 has been referenced in 1 publication.
- Smuder AJ et al. Cervical spinal cord injury exacerbates ventilator-induced diaphragm dysfunction. J Appl Physiol (1985) 120:166-77 (2016). PubMed: 26472866