Recombinant Anti-LSD2 / AOF1 antibody [EPR18508] - BSA and Azide free (ab251164)
![Western blot - Anti-LSD2 / AOF1 antibody [EPR18508] - BSA and Azide free (ab251164)](https://www.abcam.com/ps/products/251/ab251164/Images/ab251164-393686-anti-lsd2aof1-antibody-epr18508-western-blot-human-knockout-lysate.jpg "Western blot - Anti-LSD2 / AOF1 antibody [EPR18508] - BSA and Azide free (ab251164)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18508] to LSD2 / AOF1 - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra), IP, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-LSD2 / AOF1 antibody [EPR18508] - BSA and Azide free
See all LSD2 / AOF1 primary antibodies -
Description
Rabbit monoclonal [EPR18508] to LSD2 / AOF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra), IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, HAP1 and HCT116 cell lysates.
-
General notes
ab251164 is the carrier-free version of ab193080.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR18508 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
KO cell lines
-
KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
|
|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. -
Sequence similarities
Belongs to the flavin monoamine oxidase family.
Contains 1 CW-type zinc finger.
Contains 1 SWIRM domain. -
Domain
The SWIRM domain may act as an anchor site for a histone tail. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 221656 Human
- Entrez Gene: 218214 Mouse
- Entrez Gene: 306819 Rat
- Omim: 613081 Human
- SwissProt: Q8NB78 Human
- SwissProt: Q8CIG3 Mouse
- Unigene: 709336 Human
- Unigene: 31259 Mouse
-
Form
There are 3 isoforms produced by alternative splicing. -
Alternative names
- amine oxidase (flavin containing) domain 1 antibody
- amine oxidase, flavin containing 1 antibody
- AOF1 antibody
see all
Images
-
Western blot - Anti-LSD2 / AOF1 antibody [EPR18508] - BSA and Azide free (ab251164)All lanes : Anti-LSD2 / AOF1 antibody [EPR18508] (ab193080) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : KDM1B knockout HeLa cell lysate
Lane 3 : HAP1 cell lysate
Lane 4 : HCT116 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab193080).
Lanes 1-4: Merged signal (red and green). Green - ab193080 observed at 95 kDa. Red - loading control ab8245 observed at 36 kDa.
ab193080 Anti-LSD2 / AOF1 antibody [EPR18508] was shown to specifically react with LSD2 / AOF1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265969 (knockout cell lysate ab258016) was used. Wild-type and LSD2 / AOF1 knockout samples were subjected to SDS-PAGE. ab193080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Anti-LSD2 / AOF1 antibody [EPR18508] - BSA and Azide free (ab251164)](https://www.abcam.com/ps/products/251/ab251164/Images/ab251164-2-benefits-of-recombinant-antibodies.png)
Anti-LSD2 / AOF1 antibody [EPR18508] - BSA and Azide free (ab251164)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab251164 has not yet been referenced specifically in any publications.
