Recombinant Anti-LSECtin antibody [EPR13724] (ab181196)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)](https://www.abcam.com/ps/products/181/ab181196/Images/ab181196-215513-anti-lsectin-antibody-epr13724-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13724] to LSECtin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-LSECtin antibody [EPR13724]
See all LSECtin primary antibodies -
Description
Rabbit monoclonal [EPR13724] to LSECtin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver lysate and mouse liver lysates. IHC-P: Human lymph node lysate and tissue, Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13724 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 1 C-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 339390 Human
- Entrez Gene: 75863 Mouse
- Omim: 616256 Human
- SwissProt: Q6UXB4 Human
- SwissProt: Q8BNX1 Mouse
- Unigene: 220649 Human
- Unigene: 109183 Mouse
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Alternative names
- C type lectin domain family 4, member G antibody
- C type lectin superfamily 4, member G antibody
- C-type lectin domain family 4 member G antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)
Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-LSECtin antibody [EPR13724] (ab181196)](https://www.abcam.com/ps/products/181/ab181196/Images/ab181196-349269-AntiLSECtin-antibody-EPR13724-westernblot-mouse-liver.jpg)
Western blot - Anti-LSECtin antibody [EPR13724] (ab181196)Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution + Mouse liver tissue lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 180 seconds -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)](https://www.abcam.com/ps/products/181/ab181196/Images/ab181196-215512-anti-lsectin-antibody-epr13724-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (ab181196)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-LSECtin antibody [EPR13724] (ab181196)](https://www.abcam.com/ps/products/181/ab181196/Images/ab181196-215511-anti-lsectin-antibody-epr13724-western-blot.jpg)
Western blot - Anti-LSECtin antibody [EPR13724] (ab181196)All lanes : Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human lymph node lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 33 kDa -
![Anti-LSECtin antibody [EPR13724] (ab181196)](https://www.abcam.com/ps/products/181/ab181196/Images/ab181196-5-benefits-of-recombinant-antibodies.png)
Anti-LSECtin antibody [EPR13724] (ab181196)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab181196 has been referenced in 1 publication.
- Wang JB et al. An immune checkpoint score system for prognostic evaluation and adjuvant chemotherapy selection in gastric cancer. Nat Commun 11:6352 (2020). PubMed: 33311518