Recombinant Anti-LSP1 antibody [EPR5997] (ab133506)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5997] to LSP1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-LSP1 antibody [EPR5997]
See all LSP1 primary antibodies -
Description
Rabbit monoclonal [EPR5997] to LSP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human LSP1 aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Daudi, Ramos and HuT78 cell lysates. Mouse spleen and Rat brain tissue lysates.IHC-P: Human colon, Mouse spleen, Rat spleen and Human tonsil tissue. ICC/IF: Daudi Flow Cyt (intra): Daudi
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.02 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5997 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 37 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100.
For unpurified use at 1/500 - 1/1000. |
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Flow Cyt (Intra) |
1/100.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 37 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100. For unpurified use at 1/500 - 1/1000. |
Flow Cyt (Intra)
1/100. |
Target
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Function
May play a role in mediating neutrophil activation and chemotaxis. -
Tissue specificity
Activated T-lymphocytes. -
Post-translational
modificationsPhosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4046 Human
- Entrez Gene: 16985 Mouse
- Entrez Gene: 361680 Rat
- Omim: 153432 Human
- SwissProt: P33241 Human
- SwissProt: P19973 Mouse
- Unigene: 56729 Human
- Unigene: 234003 Mouse
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Alternative names
- 47 kDa actin binding protein antibody
- 47 kDa actin-binding protein antibody
- 52 kDa phosphoprotein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution (Purified) + Rat brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa -
All lanes : Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution (Purified)
Lane 1 : Mouse spleen lysates
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 3 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1:100 dilution (9.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HuT78 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab133506 at 1/250 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling LSP1 with ab133506 at 1/250 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab133506 has been referenced in 2 publications.
- Chen X et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). PubMed: 31293967
- Zhang H et al. Lymphocyte-specific protein 1 inhibits the growth of hepatocellular carcinoma by suppressing ERK1/2 phosphorylation. FEBS Open Bio 6:1227-1237 (2016). PubMed: 28255535