Recombinant Anti-LTA antibody [3F12.2D3] (ab288417)
![Western blot - Anti-LTA antibody [3F12.2D3] (ab288417)](https://www.abcam.com/ps/products/288/ab288417/Images/ab288417-479363-anti-lta-antibody-western-blot-human.JPG "Western blot - Anti-LTA antibody [3F12.2D3] (ab288417)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [3F12.2D3] to TNF beta
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-LTA antibody [3F12.2D3]
See all TNF beta primary antibodies -
Description
Mouse monoclonal [3F12.2D3] to TNF beta -
Host species
Mouse -
Specificity
3F12 binds to human LTα3 and LTαβ (with a KD of ~0.3 nM, determined by BIACORE; ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human LTA.
Database link: P01374 -
Positive control
- WB: Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3F12.2D3 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa.
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| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa. |
Target
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Function
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. -
Involvement in disease
Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated. - Information by UniProt
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Database links
- Entrez Gene: 4049 Human
- Omim: 153440 Human
- SwissProt: P01374 Human
- Unigene: 36 Human
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Alternative names
- LTalpha antibody
- DAMA-25N12.13-004 antibody
- DIF antibody
see all
Images
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Western blot - Anti-LTA antibody [3F12.2D3] (ab288417)Anti-LTA antibody [3F12.2D3] (ab288417) at 2 µg/ml + LPS-treated human peripheral blood monocyte (PBM) lysate (in RIPA buffer) at 35 µg
Predicted band size: 22 kDaThe image data was generated using a rabbit IgG-chimeric version of the same antibody clone
The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated.
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![Anti-LTA antibody [3F12.2D3] (ab288417)](https://www.abcam.com/ps/products/288/ab288417/Images/ab288417-2-benefitsofrecombinantantibodies.jpg)
Anti-LTA antibody [3F12.2D3] (ab288417)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab288417 has not yet been referenced specifically in any publications.