Recombinant Anti-LTA antibody [3F12.2D3] (ab288417)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [3F12.2D3] to TNF beta
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-LTA antibody [3F12.2D3]
See all TNF beta primary antibodies -
Description
Mouse monoclonal [3F12.2D3] to TNF beta -
Host species
Mouse -
Specificity
3F12 binds to human LTα3 and LTαβ (with a KD of ~0.3 nM, determined by BIACORE; ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms.
-
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human LTA.
Database link: P01374 -
Positive control
- WB: Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3F12.2D3 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa.
|
Notes |
---|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa. |
Target
-
Function
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. -
Involvement in disease
Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated. - Information by UniProt
-
Database links
- Entrez Gene: 4049 Human
- Omim: 153440 Human
- SwissProt: P01374 Human
- Unigene: 36 Human
-
Alternative names
- LTalpha antibody
- DAMA-25N12.13-004 antibody
- DIF antibody
see all
Images
-
Anti-LTA antibody [3F12.2D3] (ab288417) at 2 µg/ml + LPS-treated human peripheral blood monocyte (PBM) lysate (in RIPA buffer) at 35 µg
Predicted band size: 22 kDaThe image data was generated using a rabbit IgG-chimeric version of the same antibody clone
The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab288417 has not yet been referenced specifically in any publications.