Recombinant Anti-Lumican antibody [EPR8898(2)] (ab168348)
![Western blot - Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.com/ps/products/168/ab168348/Images/ab168348-168804-anti-lumican-antibody-epr88982-western-blot.jpg "Western blot - Anti-Lumican antibody [EPR8898(2)] (ab168348)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8898(2)] to Lumican
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Lumican antibody [EPR8898(2)]
See all Lumican primary antibodies -
Description
Rabbit monoclonal [EPR8898(2)] to Lumican -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Lumican aa 250-350. The exact sequence is proprietary.
Database link: P51884 -
Positive control
- WB: Saos-2, fetal skin, fetal heart, Jurkat, Y79 and HEK-293T lysates. IHC-P: Human kidney and Human skin tissues. ICC/IF: Saos-2 cells. Flow Cyt (intra): HEK-293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8898(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
|
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| IHC-P | (6) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| ICC/IF | (3) |
1/250 - 1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/250 - 1/500. |
Target
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Tissue specificity
Cornea and other tissues. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
Developmental stage
Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan. -
Post-translational
modificationsSulfated on tyrosine residue(s). -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4060 Human
- Entrez Gene: 17022 Mouse
- Entrez Gene: 81682 Rat
- Omim: 600616 Human
- SwissProt: P51884 Human
- SwissProt: P51885 Mouse
- SwissProt: P51886 Rat
- Unigene: 406475 Human
see all -
Alternative names
- Keratan sulfate proteoglycan lumican antibody
- KSPG lumican antibody
- LDC antibody
see all
Images
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Western blot - Anti-Lumican antibody [EPR8898(2)] (ab168348)All lanes : Anti-Lumican antibody [EPR8898(2)] (ab168348) at 1/1000 dilution
Lane 1 : Saos-2 lysate
Lane 2 : Fetal skin lysate
Lane 3 : Fetal heart lysate
Lane 4 : Jurkat lysate
Lane 5 : Y79 lysate
Lane 6 : 293T lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.com/ps/products/168/ab168348/Images/ab168348-168811-anti-lumican-antibody-epr88982-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (ab168348)Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Lumican with ab168348 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.com/ps/products/168/ab168348/Images/ab168348-168812-anti-lumican-antibody-epr88982-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Lumican antibody [EPR8898(2)] (ab168348)Immunofluorescent analysis of Saos-2 cells labeling Lumican with ab168348 at 1/250.
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![Flow Cytometry (Intracellular) - Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.com/ps/products/168/ab168348/Images/ab168348-168813-anti-lumican-antibody-epr88982-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Lumican antibody [EPR8898(2)] (ab168348)Intracellular flow cytometrical analysis of permeabilized 293T cells with ab168348 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.com/ps/products/168/ab168348/Images/ab168348-168810-anti-lumican-antibody-epr88982-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (ab168348)Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Lumican with ab168348 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.com/ps/products/168/ab168348/Images/ab168348-7-benefits-of-recombinant-antibodies.png)
Anti-Lumican antibody [EPR8898(2)] (ab168348)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab168348 has been referenced in 30 publications.
- Chow SE et al. Nuclear p120 catenin is a component of the perichromosomal layer and coordinates sister chromatid segregation during mitosis in lung cancer cells. Cell Death Dis 13:526 (2022). PubMed: 35660718
- Wang K et al. Lumican is elevated in the lung in human and experimental acute respiratory distress syndrome and promotes early fibrotic responses to lung injury. J Transl Med 20:392 (2022). PubMed: 36059026
- Wu J et al. Effects of lumican expression on the apoptosis of scleral fibroblasts: In vivo and in vitro experiments. Exp Ther Med 21:495 (2021). PubMed: 33791004
- Kafarnik C et al. Canine Corneal Stromal Cells Have Multipotent Mesenchymal Stromal Cell Properties In Vitro. Stem Cells Dev 29:425-439 (2020). PubMed: 31973649
- Denes BJ et al. Core Matrisome Protein Signature During Periodontal Ligament Maturation From Pre-occlusal Eruption to Occlusal Function. Front Physiol 11:174 (2020). PubMed: 32194440