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    products/primary-antibodies/lunatic-fringe-antibody-epr10391b-ab151699.pdf

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Neuroscience Neurology process Notch Pathway
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RecombinantRabMAb

Recombinant Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)

  • Datasheet
  • SDS
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Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)
  • Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)
  • Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10391(B)] to Lunatic Fringe
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Lunatic Fringe antibody [EPR10391(B)]
    See all Lunatic Fringe primary antibodies
  • Description

    Rabbit monoclonal [EPR10391(B)] to Lunatic Fringe
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • PC12, HeLa and K562 cell lysates; K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10391(B)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Cytoplasmic
    • Developmental Biology
    • Embryogenesis
    • Somatogenesis

Associated products

  • Alternative Versions

    • Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning.
  • Involvement in disease

    Defects in LFNG are the cause of spondylocostal dysostosis type 3 (SCDO3) [MIM:609813]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life.
  • Sequence similarities

    Belongs to the glycosyltransferase 31 family.
  • Post-translational
    modifications

    A soluble form may be derived from the membrane form by proteolytic processing.
  • Cellular localization

    Golgi apparatus membrane.
  • Target information above from: UniProt accession Q8NES3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3955 Human
    • Entrez Gene: 16848 Mouse
    • Entrez Gene: 170905 Rat
    • Omim: 602576 Human
    • SwissProt: Q8NES3 Human
    • SwissProt: O09010 Mouse
    • SwissProt: Q924T4 Rat
    • Unigene: 159142 Human
    • Unigene: 12834 Mouse
    • Unigene: 127809 Rat
    see all
  • Alternative names

    • 3-N-acetylglucosaminyltransferase lunatic fringe antibody
    • Beta-1 antibody
    • Beta-13-N-acetylglucosaminyltransferase lunatic fringe antibody
    • lfng antibody
    • LFNG_HUMAN antibody
    • O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase antibody
    • SCDO3 antibody
    see all

Images

  • Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)
    Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)
    All lanes : Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699) at 1/1000 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 42 kDa

  • Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)
    Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)

    Intracellular flow cytometric analysis of permeabilized K562 cells labeling Lunatic Fringe with ab151699 at 1/10 dilution (red) compared to a nonspecific control antibody (green).

     

  • Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)
    Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)

Protocols

  • Western blot protocols
  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab151699? Please let us know so that we can cite the reference in this datasheet.

ab151699 has been referenced in 1 publication.

  • Cheng W  et al. Bioinformatic analyses reveal a distinct Notch activation induced by STAT3 phosphorylation in the mesenchymal subtype of glioblastoma. J Neurosurg 126:249-259 (2017). PubMed: 26967788

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