Recombinant Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10391(B)] to Lunatic Fringe
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Lunatic Fringe antibody [EPR10391(B)]
See all Lunatic Fringe primary antibodies -
Description
Rabbit monoclonal [EPR10391(B)] to Lunatic Fringe -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- PC12, HeLa and K562 cell lysates; K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10391(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning. -
Involvement in disease
Defects in LFNG are the cause of spondylocostal dysostosis type 3 (SCDO3) [MIM:609813]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life. -
Sequence similarities
Belongs to the glycosyltransferase 31 family. -
Post-translational
modificationsA soluble form may be derived from the membrane form by proteolytic processing. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 3955 Human
- Entrez Gene: 16848 Mouse
- Entrez Gene: 170905 Rat
- Omim: 602576 Human
- SwissProt: Q8NES3 Human
- SwissProt: O09010 Mouse
- SwissProt: Q924T4 Rat
- Unigene: 159142 Human
see all -
Alternative names
- 3-N-acetylglucosaminyltransferase lunatic fringe antibody
- Beta-1 antibody
- Beta-13-N-acetylglucosaminyltransferase lunatic fringe antibody
see all
Images
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All lanes : Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699) at 1/1000 dilution
Lane 1 : PC12 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa -
Intracellular flow cytometric analysis of permeabilized K562 cells labeling Lunatic Fringe with ab151699 at 1/10 dilution (red) compared to a nonspecific control antibody (green).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151699 has been referenced in 1 publication.
- Cheng W et al. Bioinformatic analyses reveal a distinct Notch activation induced by STAT3 phosphorylation in the mesenchymal subtype of glioblastoma. J Neurosurg 126:249-259 (2017). PubMed: 26967788