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RecombinantRabMAb

Recombinant Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)

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Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)
  • Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)
  • Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10391(B)] to Lunatic Fringe - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free
    See all Lunatic Fringe primary antibodies

  • Description

    Rabbit monoclonal [EPR10391(B)] to Lunatic Fringe - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249022 is the carrier-free version of ab151699.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning.

  • Involvement in disease

    Defects in LFNG are the cause of spondylocostal dysostosis type 3 (SCDO3) [MIM:609813]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life.

  • Sequence similarities

    Belongs to the glycosyltransferase 31 family.

  • Post-translational
    modifications

    A soluble form may be derived from the membrane form by proteolytic processing.

  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 3-N-acetylglucosaminyltransferase lunatic fringe antibody
    • Beta-1 antibody
    • Beta-13-N-acetylglucosaminyltransferase lunatic fringe antibody
    • lfng antibody
    • LFNG_HUMAN antibody
    • O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase antibody
    • SCDO3 antibody
    see all

Images

  • Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)
    Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)
    All lanes : Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699) at 1/1000 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 42 kDa



    This data was developed using ab151699, the same antibody clone in a different buffer formulation.

  • Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)
    Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)

    This data was developed using ab151699, the same antibody clone in a different buffer formulation. 

    Intracellular flow cytometric analysis of permeabilized K562 cells labeling Lunatic Fringe with ab151699 at 1/10 dilution (red) compared to a nonspecific control antibody (green).

     

  • Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)
    Anti-Lunatic Fringe antibody [EPR10391(B)] - BSA and Azide free (ab249022)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab249022? Please let us know so that we can cite the reference in this datasheet.

ab249022 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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