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RecombinantRabMAb

Recombinant Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

Reviews (2) Submit a question References (47)
Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
  • Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
  • Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
  • Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6508(N)] to LXR alpha
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

Overview

  • Product name

    Anti-LXR alpha antibody [EPR6508(N)]
    See all LXR alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR6508(N)] to LXR alpha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep

  • Immunogen

    Synthetic peptide within Human LXR alpha aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13133
    (Peptide available as ab191835)

  • Positive control

    • WB: Jurkat, SH-SY5Y, IMR-32 cell non-nuclear and nuclear fractions, HEK 293 transfected with blank vectors, blank vectors non-nuclear fraction and blank vectors nuclear fraction, HEK 293 transfected with His tagged human LXR alpha full length expression vectors non-nuclear fraction and nuclear fraction, HEK 293 transfected with His tagged human LXR alpha and beta full length expression vectors.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
Application notes
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

Target

  • Function

    Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.

  • Tissue specificity

    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Images

  • Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) cells non-nuclear fraction
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) cells nuclear fraction
    Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) cells non-nuclear fraction
    Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) cells nuclear fraction
    Lane 5 : IMR-32 (Human neuroblastoma neuroblast) cells non-nuclear fraction
    Lane 6 : IMR-32 (Human neuroblastoma neuroblast) cells nuclear fraction

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 7 seconds


    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    Ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest.

    ab176842 and ab181602 were used as loading controls.

  • Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

    Lane 1 : HEK 293 transfected with blank vectors, whole cell lysate
    Lane 2 : HEK 293 transfected with blank vectors, non-nuclear fraction
    Lane 3 : HEK 293 transfected with blank vectors, nuclear fraction
    Lane 4 : HEK 293 transfected with His tagged human LXR alpha full length expression vectors, non-nuclear fraction
    Lane 5 : HEK 293 transfected with His tagged human LXR alpha full length expression vectors, nuclear fraction

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 10 seconds


    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    Ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest.

    ab213204, ab176842 and ab181602 were used as loading controls.

  • Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

    Lane 1 : HEK 293 transfected with blank vectors, whole cell lysate
    Lane 2 : HEK 293 transfected with His tagged human LXR alpha full length expression vectors, whole cell lysate
    Lane 3 : HEK 293 transfected with His tagged human LXR beta full length expression vectors, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 3 seconds


    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    Ab176323 does not cross activate with human LXR beta.

    ab213204 was used as loading control.

  • Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

References (47)

Publishing research using ab176323? Please let us know so that we can cite the reference in this datasheet.

ab176323 has been referenced in 47 publications.

  • Hori M  et al. Acute Cholesterol-Lowering Effect of Exendin-4 in Ldlr-/- and C57BL/6J Mice. J Atheroscler Thromb 30:74-86 (2023). PubMed: 35314564
  • Liu B  et al. Inhibition of Human Cytomegalovirus Particle Maturation by Activation of Liver X Receptor. Front Microbiol 13:846386 (2022). PubMed: 35330771
  • Zhao G  et al. Hydrolyzed Bound Phenolics from Rice Bran Alleviate Hyperlipidemia and Improve Gut Microbiota Dysbiosis in High-Fat-Diet Fed Mice. Nutrients 14:N/A (2022). PubMed: 35334934
  • Chen L  et al. Loss of mitochondrial ATPase ATAD3A contributes to nonalcoholic fatty liver disease through accumulation of lipids and damaged mitochondria. J Biol Chem 298:102008 (2022). PubMed: 35513069
  • Qiu R  et al. Ginsenosides Rg1 and CK Control Temozolomide Resistance in Glioblastoma Cells by Modulating Cholesterol Efflux and Lipid Raft Distribution. Evid Based Complement Alternat Med 2022:1897508 (2022). PubMed: 36276866
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-LXR alpha antibody [EPR6508(N)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Synovial membrane)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
35 µg
Specification
Synovial membrane
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 09 2016

Western blot abreview for Anti-LXR alpha antibody [EPR6508(N)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SCC61)
Gel Running Conditions
Reduced Denaturing
Loading amount
23 µg
Specification
SCC61
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 15 2015

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