Anti-LXR alpha antibody [PPZ0412] (ab41902)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902)](https://www.abcam.com/ps/products/41/ab41902/Images/ab41902-275215-anti-lxr-alpha-antibody-ppz0412-chip-grade-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902)")
Key features and details
- Mouse monoclonal [PPZ0412] to LXR alpha
- Suitable for: ICC/IF, ELISA, IP, ChIP, GSA, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LXR alpha antibody [PPZ0412]
See all LXR alpha primary antibodies -
Description
Mouse monoclonal [PPZ0412] to LXR alpha -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, ELISA, IP, ChIP, GSA, IHC-P, Flow Cytmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human LXR alpha aa 164-447.
Database link: Q13133 -
Positive control
- Rat hepatocyte and kupffer cell. Rat spleen macrophage.
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General notes
This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Physiological saline. -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
PPZ0412 -
Isotype
IgG2a -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| ELISA |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| ChIP | (1) |
Use at an assay dependent concentration.
|
| GSA |
Use at an assay dependent concentration.
|
|
| EMSA |
Use at an assay dependent concentration.
|
|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
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ChIP
Use at an assay dependent concentration. |
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GSA
Use at an assay dependent concentration. |
|
EMSA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. -
Tissue specificity
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10062 Human
- Entrez Gene: 22259 Mouse
- Entrez Gene: 58852 Rat
- Omim: 602423 Human
- SwissProt: Q13133 Human
- SwissProt: Q9Z0Y9 Mouse
- SwissProt: Q62685 Rat
- Unigene: 438863 Human
see all -
Alternative names
- Liver X receptor alpha antibody
- LXR a antibody
- LXRA antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902)This image is courtesy of an abreview submitted by Rudolf Jung, University of Regensburg.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LXR alpha with ab41902 at 1/100 dilution. Tissue was fixed with formaldehyde and permeabilized with Tween 20. Heat mediated antigen retrieval was performed using TRIS-EDTA Buffer. Tissue sections were incubated with Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) for 30 minutes at 20°C in diluent from DAKO. An undiluted HRP conjugated horse anti-mouse secondary antibody was used.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)](https://www.abcam.com/ps/products/41/ab41902/Images/ab41902-55296-anti-lxr-alpha-antibody-ppz0412-chip-grade-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)ab41902 staining LXR alpha in rat spleen macrophage cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)](https://www.abcam.com/ps/products/41/ab41902/Images/ab41902-55297-anti-lxr-alpha-antibody-ppz0412-chip-grade-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)ab41902 staining LXR alpha in Rat Hepatocyte and Kupffer cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)](https://www.abcam.com/ps/products/41/ab41902/Images/ab41902-55294-anti-lxr-alpha-antibody-ppz0412-chip-grade-flow-cytometry.jpg)
Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)Overlay histogram showing HepG2 cells stained with ab41902 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41902, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (78)
ab41902 has been referenced in 78 publications.
- Chinetti G et al. Diabetes-Induced Changes in Macrophage Biology Might Lead to Reduced Risk for Abdominal Aortic Aneurysm Development. Metabolites 12:N/A (2022). PubMed: 35208203
- Lin CY et al. The Citrus Flavonoid Nobiletin Downregulates Angiopoietin-like Protein 3 (ANGPTL3) Expression and Exhibits Lipid-Modulating Effects in Hepatic Cells and Adult Zebrafish Models. Int J Mol Sci 23:N/A (2022). PubMed: 36293338
- Gao WY et al. Tanshinone IIA Downregulates Lipogenic Gene Expression and Attenuates Lipid Accumulation through the Modulation of LXRa/SREBP1 Pathway in HepG2 Cells. Biomedicines 9:N/A (2021). PubMed: 33806955
- Pu Y et al. MicroRNA-325 facilitates atherosclerosis progression by mediating the SREBF1/LXR axis via KDM1A. Life Sci 277:119464 (2021). PubMed: 33811891
- Zhang X et al. Tumor Suppressor Gene XEDAR Promotes Differentiation and Suppresses Proliferation and Migration of Gastric Cancer Cells Through Upregulating the RELA/LXRα Axis and Deactivating the Wnt/β-Catenin Pathway. Cell Transplant 30:963689721996346 (2021). PubMed: 33637015