Recombinant Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4445] to LYRIC/AEG1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-LYRIC/AEG1 antibody [EP4445]
See all LYRIC/AEG1 primary antibodies -
Description
Rabbit monoclonal [EP4445] to LYRIC/AEG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human LYRIC/AEG1 aa 250-350. The exact sequence is proprietary.
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Positive control
- HeLa, Jurkat, PC3, PC12 and NIH 3T3 cell lysates; Human brain and breast tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP4445 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
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ICC/IF |
1/500.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
ICC/IF
1/500. |
Target
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Function
Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance. -
Tissue specificity
Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme. -
Cellular localization
Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 92140 Human
- Entrez Gene: 67154 Mouse
- Entrez Gene: 170910 Rat
- Omim: 610323 Human
- SwissProt: Q86UE4 Human
- SwissProt: Q80WJ7 Mouse
- SwissProt: Q9Z1W6 Rat
- Unigene: 377155 Human
see all -
Alternative names
- 3D3 antibody
- 3D3/LYRIC antibody
- AEG 1 antibody
see all
Images
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All lanes : Anti-LYRIC/AEG1 antibody [EP4445] (ab124789) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : PC3 cell lysate
Lane 4 : PC12 cell lysate
Lane 5 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 64 kDa -
ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human breast tissues by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LYRIC/AEG1 with purified ab124789 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Control: PBS only.
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ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human brain tissues by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab124789 has been referenced in 14 publications.
- Wei W et al. LOC102553417 silencing facilitates the apoptosis of hepatic stellate cells via the miR‑30e/MTDH axis. Mol Med Rep 26:N/A (2022). PubMed: 36177898
- Deng J et al. Long Noncoding RNA DLGAP1-AS1 Promotes the Aggressive Behavior of Gastric Cancer by Acting as a ceRNA for microRNA-628-5p and Raising Astrocyte Elevated Gene 1 Expression. Cancer Manag Res 12:2947-2960 (2020). PubMed: 32431541
- Fan H et al. Long Non-Coding RNA DARS-AS1 Contributes to Prostate Cancer Progression Through Regulating the MicroRNA-628-5p/MTDH Axis. Cancer Manag Res 12:8363-8377 (2020). PubMed: 32982430
- Wang F miR-384 targets metadherin gene to suppress growth, migration, and invasion of gastric cancer cells. J Int Med Res 47:926-935 (2019). PubMed: 30614349
- Zhu YZ et al. Metadherin overexpression in perihilar cholangiocarcinoma is associated with lymph node metastasis and poor prognosis. Oncol Lett 17:4514-4520 (2019). PubMed: 30988817