RecombinantRabMAb

Recombinant Anti-M-CSF antibody [EPR20948] (ab233387)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)
  • Western blot - Anti-M-CSF antibody [EPR20948] (ab233387)
  • Western blot - Anti-M-CSF antibody [EPR20948] (ab233387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)
  • Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EPR20948] (ab233387)
  • Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EPR20948] (ab233387)
  • Anti-M-CSF antibody [EPR20948] (ab233387)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20948] to M-CSF
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

Overview

  • Product name

    Anti-M-CSF antibody [EPR20948]
    See all M-CSF primary antibodies

  • Description

    Rabbit monoclonal [EPR20948] to M-CSF

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U937, P388D1, J774A.1, Jurkat, MDA-MB-231, K562, C6, PC-12 and NIH/3T3 whole cell lysates; Human tonsil, brain and kidney lysates. IHC-P: Human colon and bladder cancer tissues; Mouse and rat colon tissues. ICC/IF: Jurkat cells. Flow Cyt (intra): Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab233387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.

Target

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.

  • Post-translational
    modifications

    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.

  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Colony stimulating factor 1 (macrophage) antibody
    • Colony stimulating factor 1 antibody
    • Colony stimulating factor macrophage specific antibody
    • CSF 1 antibody
    • CSF-1 antibody
    • CSF1 antibody
    • CSF1_HUMAN antibody
    • Csfm antibody
    • Lanimostim antibody
    • M CSF antibody
    • M-CSF antibody
    • Macrophage Colony Stimulating Factor 1 antibody
    • Macrophage colony stimulating factor antibody
    • MCSF antibody
    • MGC31930 antibody
    • Processed macrophage colony-stimulating factor 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of human colon (PMID: 15519852; PMID: 11745698).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Western blot - Anti-M-CSF antibody [EPR20948] (ab233387)
    Western blot - Anti-M-CSF antibody [EPR20948] (ab233387)
    All lanes : Anti-M-CSF antibody [EPR20948] (ab233387) at 1/1000 dilution

    Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
    Lane 2 : P388D1 (mouse lymphoma monocyte; macrophage cell line) whole cell lysate
    Lanes 3 & 7 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage cell line) whole cell lysate
    Lane 4 : Human tonsil lysate
    Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 6 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 60 kDa
    Observed band size: 43 kDa why is the actual band size different from the predicted?



    Exposure time : Lanes 1-4: 3 minutes; Lanes 5: 6 seconds; Lanes 6-8: 26 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21502940; PMID: 3264877).

  • Western blot - Anti-M-CSF antibody [EPR20948] (ab233387)
    Western blot - Anti-M-CSF antibody [EPR20948] (ab233387)
    All lanes : Anti-M-CSF antibody [EPR20948] (ab233387) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 4 : Human brain lysate
    Lane 5 : Human kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 60 kDa
    Observed band size: 43 kDa why is the actual band size different from the predicted?



    Exposure time : Lanes 1-3: 3 minutes; Lanes 4-5:10 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 21502940; PMID: 3264877).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.  Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Membranous and weak cytoplasmic staining on human bladder cancer (PMID: 25082815; PMID: 25667468) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of mouse colon (PMID: 15519852; PMID: 11745698).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of rat colon (PMID: 15519852; PMID: 11745698)

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EPR20948] (ab233387)
    Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EPR20948] (ab233387)

    Immunofluorescent analysis of 100% methanol-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling M-CSF with ab233387 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Jurkat cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EPR20948] (ab233387)
    Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EPR20948] (ab233387)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling M-CSF with ab233387 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Anti-M-CSF antibody [EPR20948] (ab233387)
    Anti-M-CSF antibody [EPR20948] (ab233387)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (6)

Publishing research using ab233387? Please let us know so that we can cite the reference in this datasheet.

ab233387 has been referenced in 6 publications.

  • Beuker C  et al. Stroke induces disease-specific myeloid cells in the brain parenchyma and pia. Nat Commun 13:945 (2022). PubMed: 35177618
  • Patel RR  et al. Ethanol withdrawal-induced adaptations in prefrontal corticotropin releasing factor receptor 1-expressing neurons regulate anxiety and conditioned rewarding effects of ethanol. Mol Psychiatry 27:3441-3451 (2022). PubMed: 35668157
  • Liu X  et al. Construction of the Prediction Model for Locally Advanced Rectal Cancer Following Neoadjuvant Chemoradiotherapy Based on Pretreatment Tumor-Infiltrating Macrophage-Associated Biomarkers. Onco Targets Ther 14:2599-2610 (2021). PubMed: 33880038
  • Zhang S  et al. Activation of Toll-Like Receptor 7 Signaling Pathway in Primary Sjögren's Syndrome-Associated Thrombocytopenia. Front Immunol 12:637659 (2021). PubMed: 33767707
  • Chen J  et al. IP10, KC and M-CSF Are Remarkably Increased in the Brains from the Various Strains of Experimental Mice Infected with Different Scrapie Agents. Virol Sin 35:614-625 (2020). PubMed: 32314275
  • Ren C  et al. An effective and biocompatible polyethylenimine based vaginal suppository for gene delivery. Nanomedicine 20:101994 (2019). PubMed: 31028885

Customer reviews and Q&As

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Immunohistochemistry free floating abreview for Anti-M-CSF antibody [EPR20948]

 Average
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Forebrain)
Specification
Forebrain
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Mar 18 2021

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