Anti-Mannan Binding Lectin/MBL antibody [11C9] (ab26277)
Key features and details
- Mouse monoclonal [11C9] to Mannan Binding Lectin/MBL
- Suitable for: ELISA, IP, WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Mannan Binding Lectin/MBL antibody [11C9]
See all Mannan Binding Lectin/MBL primary antibodies -
Description
Mouse monoclonal [11C9] to Mannan Binding Lectin/MBL -
Host species
Mouse -
Specificity
ab26277 reacts with human Mannan binding lectin/MBL both in its polymeric state and as a single subunit of molecular mass 26 kDa. It reacts with Mannan binding lectin/MBL from serum and plasma.
-
Tested applications
Suitable for: ELISA, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Mannan Binding Lectin/MBL.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
Purification notes
Protein A/G purified -
Clonality
Monoclonal -
Clone number
11C9 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
Target
-
Function
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
Tissue specificity
Plasma protein produced mainly in the liver. -
Involvement in disease
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 4153 Human
- Omim: 154545 Human
- SwissProt: P11226 Human
- Unigene: 499674 Human
-
Alternative names
- COLEC 1 antibody
- COLEC1 antibody
- Collectin-1 antibody
see all
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab26277 has been referenced in 6 publications.
- Liu Y et al. MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and Fibrosis. J Inflamm Res 15:4315-4329 (2022). PubMed: 35923908
- Bhute VJ et al. Mannose Binding Lectin Is Hydroxylated by Collagen Prolyl-4-hydroxylase and Inhibited by Some PHD Inhibitors. Kidney360 1:447-457 (2020). PubMed: 35368589
- Bibert S et al. Herpes simplex encephalitis in adult patients with MASP-2 deficiency. PLoS Pathog 15:e1008168 (2019). PubMed: 31869396
- Harboe M et al. Design of a complement mannose-binding lectin pathway-specific activation system applicable at low serum dilutions. Clin Exp Immunol 144:512-20 (2006). PubMed: 16734621
- Larsen F et al. Disease-associated mutations in human mannose-binding lectin compromise oligomerization and activity of the final protein. J Biol Chem 279:21302-11 (2004). PubMed: 14764589
- Downing I et al. Immature dendritic cells possess a sugar-sensitive receptor for human mannan-binding lectin. Immunology 109:360-4 (2003). PubMed: 12807481