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Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)

Reviews (2)Q&A (3)References (17)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)

Key features and details

  • Mouse monoclonal [3B6] to Mannan Binding Lectin/MBL
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Mannan Binding Lectin/MBL antibody [3B6]
    See all Mannan Binding Lectin/MBL primary antibodies

  • Description

    Mouse monoclonal [3B6] to Mannan Binding Lectin/MBL

  • Host species

    Mouse

  • Specificity

    ab23457 is specific for MBL from human serum or plasma. In Western blotting, ab121075 reacts with Human MBL in both its oligomerized state.

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human Mannan Binding Lectin/MBL. Human MBL purified from Human donor plasma.

  • Epitope

    The epitope is on the head-neck region of the MBL protein chain.

  • Positive control

    • Human liver, Human cancerous liver and Human brain tissues.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    3B6

  • Myeloma

    x63-Ag8.653

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab23457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/250.
Notes
IHC-P
1/250.

Target

  • Function

    Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

  • Tissue specificity

    Plasma protein produced mainly in the liver.

  • Involvement in disease

    Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.

  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • COLEC 1 antibody
      • COLEC1 antibody
      • Collectin-1 antibody
      • HSMBPC antibody
      • Lectin, mannose-binding, soluble, 2 antibody
      • Mannan binding lectin antibody
      • Mannan binding protein antibody
      • Mannan-binding protein antibody
      • Mannose binding lectin (protein C) 2 soluble antibody
      • Mannose binding lectin (protein C) 2, soluble (opsonic defect) antibody
      • Mannose binding lectin (protein C) 2, soluble antibody
      • Mannose binding lectin 2 soluble antibody
      • Mannose binding lectin 2, soluble (opsonic defect) antibody
      • Mannose binding lectin antibody
      • Mannose binding lectin protein C2 soluble opsonic defect antibody
      • Mannose binding protein antibody
      • Mannose binding protein C antibody
      • Mannose binding protein C precursor antibody
      • Mannose binding protein, serum antibody
      • Mannose-binding lectin antibody
      • Mannose-binding protein C antibody
      • MBL 2 antibody
      • MBL antibody
      • MBL2 antibody
      • MBL2_HUMAN antibody
      • MBL2D antibody
      • MBP 1 antibody
      • MBP antibody
      • MBP C antibody
      • MBP-C antibody
      • MBP1 antibody
      • MBPB antibody
      • MBPC antibody
      • MBPD antibody
      • MGC116832 antibody
      • MGC116833 antibody
      • Opsonic defect antibody
      • protein C antibody
      • Soluble mannose binding lectin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
      ab23457 at 1 mg/ml staining human liver by IHC-P
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)

      ab23457 staining MBL in Paraffin-embedded Human Liver tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)

      ab23457 staining MBL in Paraffin-embedded Human cancerous liver tissue by Immunohistochemistry.

    References (17)

    Publishing research using ab23457? Please let us know so that we can cite the reference in this datasheet.

    ab23457 has been referenced in 17 publications.

    • Jiao Y  et al. Activation of complement C1q and C3 in glomeruli might accelerate the progression of diabetic nephropathy: Evidence from transcriptomic data and renal histopathology. J Diabetes Investig 13:839-849 (2022). PubMed: 34932275
    • Jiang S  et al. Activation of Complement Pathways in Kidney Tissue May Mediate Tubulointerstitial Injury in Diabetic Nephropathy. Front Med (Lausanne) 9:845679 (2022). PubMed: 35479942
    • Liu Y  et al. MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and Fibrosis. J Inflamm Res 15:4315-4329 (2022). PubMed: 35923908
    • Haddad G  et al. Altered glycosylation of IgG4 promotes lectin complement pathway activation in anti-PLA2R1-associated membranous nephropathy. J Clin Invest 131:N/A (2021). PubMed: 33351779
    • Yadav AK  et al. Differential expression of collectins in human placenta and role in inflammation during spontaneous labor. PLoS One 9:e108815 (2014). WB ; Human . PubMed: 25303045
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannan Binding Lectin/MBL antibody [3B6]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 22 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannan Binding Lectin/MBL antibody [3B6]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain tissue)
    Antigen retrieval step
    Heat mediated
    Specification
    brain tissue
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Vlady Pavlova

    Verified customer

    Submitted Aug 15 2006

    Question

    The placental proteins were harvested using the lysis buffer containing 8M Urea and 2%CHAPS supplemented with protein inhibitors. Protein samples were prepared using Laemalli buffer with beta-ME and DTT, boiled for five minutes. The proteins were resolved on 10%SDS-PAGE and in reducing conditions. The membrane was blocked in 1% non fat dry milk in TBS over night at 4 degree. The primary antibody incubation was 1:30hr to 2:00 hr at room temperature. The membrane was washed for 3-4 times (10 min/wash) using TBST (Triton X-100, 0.02%). Secondary antibody was Goat polyclonal anti mouse IgG-HRP (1:1000 dilution), incubation time 1:00 hr at room temperature. The antibody signals were detected using ECL method.

    Read More
    Answer

    Thank you for this additional information. A few suggestions just to follow a protocol a little more similar to how we have tested. We generally recommend blocking in 5% milk in TBST and incubating the primary antibody overnight at 4 degrees. You may also wish to try changing your lysis buffer to RIPA if possible.



    At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund within 6 months of purchase.

    The Abcam Abpromise® guarantee:
    - 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors.
    - We guarantee our products work in the tested species and applications stated on the datasheet.
    - We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase.
    - We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards.

    Please note these conditions to our Abpromise®:
    - Protocol information must be provided in order for the claim to be validated.
    - Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples.
    - “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed.
    - Fast Track antibodies are covered for ELISA against the immunizing peptide only.

    I hope this information is helpful, but please do not hesitate to contact us with further questions.

    Read More

    Question

    My lab has purchased one of your Mabs against mannose binding protein/lectin (MBL)(ab23457). Are there tissue staining results from epidermis available for any of your anti-MBL Mabs? Further do you sell human MBL? Do you know of a source for human MBL?

    Read More
    Answer

    Thank you for your enquiry. The antibody has been tested in IHC in human liver and brain sections, but we do not have those images unfortunately. The reference Holmskov UL Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59 (2000) has used the antibody in immunohistochemistry and should have some images with this antibody. We currently do not sell recombinant human MBL and unfortunately I do not know of a source of such a product. You may be able to find this information from the website www.biocompare.com or the supplier Sigma,

    Read More

    Question

    I have ordered product ab23457 (mouse monoclonal to mannan binding lectin), and would like to label it. Unfortunately, the buffer it is supplied in contains 15mM azide, which is inhibitory to labeling by succinimydyl esters (according to molecular probes/invitrogen). I would like to use dialysis to remove the excess azide - however, I do not know the precise composition of the buffer the antibody was shipped in (which I need to formulate the buffer to dialyze against). Could you provide me with this information?

    Read More
    Answer

    Thank you for your enquiry. I have been informed by our lab that the antiserum was prepared in 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide. Please do not hesitate to contact me should you require further assistance.

    Read More

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