Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)](https://www.abcam.com/ps/products/23/ab23457/Images/ab23457-33311-anti-mannan-binding-lectin-antibody-3b6-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)")
Key features and details
- Mouse monoclonal [3B6] to Mannan Binding Lectin/MBL
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Mannan Binding Lectin/MBL antibody [3B6]
See all Mannan Binding Lectin/MBL primary antibodies -
Description
Mouse monoclonal [3B6] to Mannan Binding Lectin/MBL -
Host species
Mouse -
Specificity
ab23457 is specific for MBL from human serum or plasma. In Western blotting, ab121075 reacts with Human MBL in both its oligomerized state. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Mannan Binding Lectin/MBL. Human MBL purified from Human donor plasma.
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Epitope
The epitope is on the head-neck region of the MBL protein chain. -
Positive control
- Human liver, Human cancerous liver and Human brain tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3B6 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
1/250.
|
| Notes |
|---|
|
IHC-P
1/250. |
Target
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Function
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
Tissue specificity
Plasma protein produced mainly in the liver. -
Involvement in disease
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4153 Human
- Omim: 154545 Human
- SwissProt: P11226 Human
- Unigene: 499674 Human
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Alternative names
- COLEC 1 antibody
- COLEC1 antibody
- Collectin-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)ab23457 at 1 mg/ml staining human liver by IHC-P
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)](https://www.abcam.com/ps/products/23/ab23457/Images/ab23457-229226-anti-mannan-binding-lectin-antibody-3b6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)ab23457 staining MBL in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)](https://www.abcam.com/ps/products/23/ab23457/Images/ab23457-229225-anti-mannan-binding-lectin-antibody-3b6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)ab23457 staining MBL in Paraffin-embedded Human cancerous liver tissue by Immunohistochemistry.
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab23457 has been referenced in 17 publications.
- Jiao Y et al. Activation of complement C1q and C3 in glomeruli might accelerate the progression of diabetic nephropathy: Evidence from transcriptomic data and renal histopathology. J Diabetes Investig 13:839-849 (2022). PubMed: 34932275
- Jiang S et al. Activation of Complement Pathways in Kidney Tissue May Mediate Tubulointerstitial Injury in Diabetic Nephropathy. Front Med (Lausanne) 9:845679 (2022). PubMed: 35479942
- Liu Y et al. MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and Fibrosis. J Inflamm Res 15:4315-4329 (2022). PubMed: 35923908
- Haddad G et al. Altered glycosylation of IgG4 promotes lectin complement pathway activation in anti-PLA2R1-associated membranous nephropathy. J Clin Invest 131:N/A (2021). PubMed: 33351779
- Yadav AK et al. Differential expression of collectins in human placenta and role in inflammation during spontaneous labor. PLoS One 9:e108815 (2014). WB ; Human . PubMed: 25303045