Recombinant Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060)
![Western blot - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060)](https://www.abcam.com/ps/products/212/ab212060/Images/ab212060-313134-anti-mannan-binding-lectin-antibody-epr18381-83-western-blot.jpg "Western blot - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18381-83] to Mannan Binding Lectin/MBL
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83]
See all Mannan Binding Lectin/MBL primary antibodies -
Description
Rabbit monoclonal [EPR18381-83] to Mannan Binding Lectin/MBL -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse serum; Mouse kidney lysate. IHC-P: Mouse liver tissue. IP: Mouse serum.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18381-83 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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| IP |
1/30.
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| Notes |
|---|
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IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
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IP
1/30. |
Target
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Function
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
Tissue specificity
Plasma protein produced mainly in the liver. -
Involvement in disease
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 17195 Mouse
- SwissProt: P41317 Mouse
- Unigene: 30045 Mouse
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Alternative names
- COLEC 1 antibody
- COLEC1 antibody
- Collectin-1 antibody
see all
Images
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Western blot - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060)All lanes : Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Line 1: 3 seconds: Lane 2: 41 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060)](https://www.abcam.com/ps/products/212/ab212060/Images/ab212060-313135-anti-mannan-binding-lectin-antibody-epr18381-83-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Mannan Binding Lectin/MBL with ab212060 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse liver (PMID: 12538708) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060)](https://www.abcam.com/ps/products/212/ab212060/Images/ab212060-313136-anti-mannan-binding-lectin-antibody-epr18381-83-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060)Mannan Binding Lectin/MBL was immunoprecipitated from 0.35 mg of mouse serum with ab212060 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab212060 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution
Lane 1: Mouse serum 10 µg (Input).
Lane 2: ab212060 IP in mouse serum.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab212060 in mouse serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060)](https://www.abcam.com/ps/products/212/ab212060/Images/ab212060-7-benefits-of-recombinant-antibodies.png)
Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab212060 has not yet been referenced specifically in any publications.