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    products/primary-antibodies/map2-antibody-ab5392.pdf

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Anti-MAP2 antibody (ab5392)

  • Datasheet
  • SDS
Reviews (63)Q&A (14)References (681)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Western blot - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Western blot - Anti-MAP2 antibody (ab5392)

Key features and details

  • Chicken polyclonal to MAP2
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgY

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-MAP2 antibody
    See all MAP2 primary antibodies
  • Description

    Chicken polyclonal to MAP2
  • Host species

    Chicken
  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human MAP2. Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.

  • Positive control

    • IHC-P: Rat cerebellum tissue. ICC: Rat E20 cultured cortical neuron-glial cells, rat cortical neurons, rat cortical neurons and glia in mixed tissue culture and mouse neurons. WB: Adult rat brain, Embryonic E20 rat brain and Adult mouse brain lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.032% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purification notes

    Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Signal Transduction
    • Antibodies
    • map2

Associated products

  • Compatible Secondaries

    • Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
    • Goat Anti-Chicken IgY H&L (HRP) (ab6877)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant Human MAP2 protein (ab114686)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    • Anti-Alpha-synuclein antibody [LB 509] (ab27766)
    • Rat MAP2 peptide (ab32453)
    • Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC (4)
1/5000 - 1/10000.
IHC-P (13)
1/10000.
WB (6)
1/20000 - 1/100000.

This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.

Notes
ICC
1/5000 - 1/10000.
IHC-P
1/10000.
WB
1/20000 - 1/100000.

This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.

Target

  • Function

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities

    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Target information above from: UniProt accession P11137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 17756 Mouse
    • Entrez Gene: 25595 Rat
    • SwissProt: P20357 Mouse
    • SwissProt: P15146 Rat
    • Unigene: 256966 Mouse
    • Unigene: 436793 Mouse
    • Unigene: 10484 Rat
    • Unigene: 136301 Rat
    • Unigene: 230443 Rat
    see all
  • Alternative names

    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)

    Chromogenic Immunostaining of a formalin fixed paraffin embedded rat cerebellum section with chicken pAb to MAP2A/B dilution 1/10000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. ab5392 detects neuronal cells, specifically their perikarya and dendrites. Note the prominent staining of Purkinje cells and their dendrites in the molecular layer and staining of the much smaller granule neurons in the granular layer.

  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)

    Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.

  • Western blot - Anti-MAP2 antibody (ab5392)
    Western blot - Anti-MAP2 antibody (ab5392)
    All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution

    Lane 1 : Adult rat brain lysate
    Lane 2 : Embryonic E20 rat brain lysate
    Lane 3 : Adult mouse brain lysate
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreview
    ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-MAP2 antibody (ab5392)
    Western blot - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreview
    Anti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg

    Secondary
    HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 280,70 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

Protocols

  • Recommended IF (ICC) protocol with ab5392

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (681)

Publishing research using ab5392? Please let us know so that we can cite the reference in this datasheet.

ab5392 has been referenced in 681 publications.

  • Brunner JW  et al. Power and optimal study design in iPSC-based brain disease modelling. Mol Psychiatry 28:1545-1556 (2023). PubMed: 36385170
  • Jorratt P  et al. Endogenous Modulators of NMDA Receptor Control Dendritic Field Expansion of Cortical Neurons. Mol Neurobiol 60:1440-1452 (2023). PubMed: 36462136
  • Limone F  et al. Efficient generation of lower induced motor neurons by coupling Ngn2 expression with developmental cues. Cell Rep 42:111896 (2023). PubMed: 36596304
  • Pietiläinen O  et al. Astrocytic cell adhesion genes linked to schizophrenia correlate with synaptic programs in neurons. Cell Rep 42:111988 (2023). PubMed: 36640364
  • Xu J  et al. Lactate attenuates astrocytic inflammation by inhibiting ubiquitination and degradation of NDRG2 under oxygen-glucose deprivation conditions. J Neuroinflammation 19:314 (2022). PubMed: 36572898
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Immunocytochemistry abreview for Anti-MAP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (iPSC-NSC)
Permeabilization
Yes - 0.5% triton
Specification
iPSC-NSC
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 16 2021

Immunocytochemistry abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (NSCs (Neural Stem cells))
Permeabilization
Yes - tritonx100
Specification
NSCs (Neural Stem cells)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Kyungjoo Seong

Verified customer

Submitted Nov 02 2016

Immunocytochemistry abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Cell (Adipose tissue)
Specification
Adipose tissue
Permeabilization
Yes - 0.3% TritonX-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Hye Yeong Lee

Verified customer

Submitted Mar 16 2015

Immunocytochemistry abreview for Anti-MAP2 antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cell (primary hippocampal neurons)
Specification
primary hippocampal neurons
Fixative
4% PFA/Sucrose
Permeabilization
Yes - 0,1% Saponin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 09 2010

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