Anti-MAP2 antibody (ab5392)
Key features and details
- Chicken polyclonal to MAP2
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgY
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- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MAP2 antibody
See all MAP2 primary antibodies -
Description
Chicken polyclonal to MAP2 -
Host species
Chicken -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human MAP2. Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.
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Positive control
- IHC-P: Rat cerebellum tissue. ICC: Rat E20 cultured cortical neuron-glial cells, rat cortical neurons, rat cortical neurons and glia in mixed tissue culture and mouse neurons. WB: Adult rat brain, Embryonic E20 rat brain and Adult mouse brain lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.032% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purification notes
Lipid extraction from egg yolk, followed by ammonium sulphate precipitation. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | (4) |
1/5000 - 1/10000.
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| IHC-P | (13) |
1/10000.
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| WB | (6) |
1/20000 - 1/100000.
This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
| Notes |
|---|
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ICC
1/5000 - 1/10000. |
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IHC-P
1/10000. |
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WB
1/20000 - 1/100000. This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
Target
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Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
Sequence similarities
Contains 3 Tau/MAP repeats. -
Post-translational
modificationsPhosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 256966 Mouse
- Unigene: 436793 Mouse
- Unigene: 10484 Rat
- Unigene: 136301 Rat
see all -
Alternative names
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)
Chromogenic Immunostaining of a formalin fixed paraffin embedded rat cerebellum section with chicken pAb to MAP2A/B dilution 1/10000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. ab5392 detects neuronal cells, specifically their perikarya and dendrites. Note the prominent staining of Purkinje cells and their dendrites in the molecular layer and staining of the much smaller granule neurons in the granular layer.
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Immunocytochemistry - Anti-MAP2 antibody (ab5392)Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.
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Western blot - Anti-MAP2 antibody (ab5392)All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution
Lane 1 : Adult rat brain lysate
Lane 2 : Embryonic E20 rat brain lysate
Lane 3 : Adult mouse brain lysate -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi. -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue). -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreviewab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody. -
Western blot - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous AbreviewAnti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg
Secondary
HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 280,70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Datasheets and documents
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SDS download
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Datasheet download
References (681)
ab5392 has been referenced in 681 publications.
- Brunner JW et al. Power and optimal study design in iPSC-based brain disease modelling. Mol Psychiatry 28:1545-1556 (2023). PubMed: 36385170
- Jorratt P et al. Endogenous Modulators of NMDA Receptor Control Dendritic Field Expansion of Cortical Neurons. Mol Neurobiol 60:1440-1452 (2023). PubMed: 36462136
- Limone F et al. Efficient generation of lower induced motor neurons by coupling Ngn2 expression with developmental cues. Cell Rep 42:111896 (2023). PubMed: 36596304
- Pietiläinen O et al. Astrocytic cell adhesion genes linked to schizophrenia correlate with synaptic programs in neurons. Cell Rep 42:111988 (2023). PubMed: 36640364
- Xu J et al. Lactate attenuates astrocytic inflammation by inhibiting ubiquitination and degradation of NDRG2 under oxygen-glucose deprivation conditions. J Neuroinflammation 19:314 (2022). PubMed: 36572898