Recombinant Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)
![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.com/ps/products/53/ab53277/Images/ab53277-395392-anti-mapk6erk-3-antibody-ep1720y-western-blot-human-knockout-lysate.jpg "Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1720Y] to MAPK6/ERK3
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MAPK6/ERK3 antibody [EP1720Y]
See all MAPK6/ERK3 primary antibodies -
Description
Rabbit monoclonal [EP1720Y] to MAPK6/ERK3 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MAPK6/ERK3 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q16659 -
Positive control
- WB: Wild-type HAP1, A431, C2C12, 2.4G2 and HeLa (ab150035) whole cell lysates, and rat brain tissue lysates. IHC-P: Human kidney carcinoma and breast carcinoma tissues.
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General notes
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1720Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 63 kDa).
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| IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/50 |
| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 63 kDa). |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/50 |
Target
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Function
Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle. -
Tissue specificity
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme. - Information by UniProt
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Database links
- Entrez Gene: 5597 Human
- Entrez Gene: 50772 Mouse
- Entrez Gene: 58840 Rat
- Omim: 602904 Human
- SwissProt: Q16659 Human
- SwissProt: Q61532 Mouse
- SwissProt: P27704 Rat
- Unigene: 411847 Human
see all -
Alternative names
- ERK-3 antibody
- ERK3 antibody
- Extracellular signal regulated kinase 3 antibody
see all
Images
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Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)All lanes : Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MAPK6 knockout HeLa cell lysate
Lane 3 : 2.4G2 (Rat B cell lymphoma B lymphocyte) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab53277 observed at 90 kDa. Red - loading control ab8245 observed at 36 kDa.
ab53277 Anti-MAPK6/ERK3 antibody [EP1720Y] was shown to specifically react with MAPK6/ERK3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264910 (knockout cell lysate ab257526) was used. Wild-type and MAPK6/ERK3 knockout samples were subjected to SDS-PAGE. ab53277 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.com/ps/products/53/ab53277/Images/ab53277-5-anti-mapk6-erk-3-antibody-ep1720y-immunohistochemistry-kidney-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling MAPK6/ERK3 with Purified ab53277 at 1:100 dilution (13.52 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.com/ps/products/53/ab53277/Images/ab53277-369828-anti-mapk6erk-3-antibody-ep1720y-western-blot-hap1-wt-hap1-mapk6-knockout-hap1-ccne2-knockout-a431.jpg)
Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)All lanes : Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MAPK6 knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab53277 observed at 90 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab53277 was shown to react with MAPK6 in HAP1 wild-type cells in Western blot. Loss of signal was observed when MAPK6 knockout sample was used. HAP1 wild-type and MAPK6 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab53277 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.com/ps/products/53/ab53277/Images/ab53277-367562-anti-mapk6-erk-3-antibody-ep1720y-westernblot-human-mouse-rat.jpg)
Western blot - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)All lanes : Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Rat brain lysates
Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in PMID:30642948 and 30166347
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.com/ps/products/53/ab53277/Images/ab53277-66048-anti-mapk6-erk-3-antibody-ep1720y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)Unpurified ab53277 (1:50) staining human MAPK6/ERK3 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)](https://www.abcam.com/ps/products/53/ab53277/Images/ab53277-8-benefits-of-recombinant-antibodies.png)
Anti-MAPK6/ERK3 antibody [EP1720Y] (ab53277)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab53277 has been referenced in 21 publications.
- Alshammari ES et al. ERK3 is transcriptionally upregulated by ?Np63a and mediates the role of ?Np63a in suppressing cell migration in non-melanoma skin cancers. BMC Cancer 21:155 (2021). PubMed: 33579235
- Guo YP et al. Suppression of lncRNA HOTAIR alleviates RCC angiogenesis through regulating miR-126/EGFL7 axis. Am J Physiol Cell Physiol 320:C880-C891 (2021). PubMed: 33502949
- Wang H et al. MicroRNA-490-3p suppresses the proliferation and invasion of hepatocellular carcinoma cells via targeting TMOD3. Oncol Lett 20:95 (2020). PubMed: 32831914
- Li G et al. Exosomes-carried microRNA-26b-5p regulates microglia M1 polarization after cerebral ischemia/reperfusion. Cell Cycle 19:1022-1035 (2020). PubMed: 32208888
- Wang Y et al. GRHL2 induces liver fibrosis and intestinal mucosal barrier dysfunction in non-alcoholic fatty liver disease via microRNA-200 and the MAPK pathway. J Cell Mol Med 24:6107-6119 (2020). PubMed: 32324317