Anti-MAPRE1/EB1 antibody [KT51] (ab53358)
![Flow Cytometry - Anti-MAPRE1/EB1 antibody [KT51] (ab53358)](https://www.abcam.com/ps/products/53/ab53358/Images/ab53358-66137-anti-mapre1-antibody-kt51-flow-cytometry.jpg "Flow Cytometry - Anti-MAPRE1/EB1 antibody [KT51] (ab53358)")
Key features and details
- Rat monoclonal [KT51] to MAPRE1/EB1
- Suitable for: IP, WB, ICC, ELISA, ICC/IF, Flow Cyt
- Reacts with: Mouse, Hamster, Human
- Isotype: IgG2a
Overview
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Product name
Anti-MAPRE1/EB1 antibody [KT51]
See all MAPRE1/EB1 primary antibodies -
Description
Rat monoclonal [KT51] to MAPRE1/EB1 -
Host species
Rat -
Tested applications
Suitable for: IP, WB, ICC, ELISA, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Hamster, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KT51 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
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| WB | (2) |
Use at an assay dependent concentration.
|
| ICC | (1) |
Use at an assay dependent concentration.
|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use 1µg for 106 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
Domain
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome. - Information by UniProt
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Database links
- Entrez Gene: 22919 Human
- Entrez Gene: 13589 Mouse
- Omim: 603108 Human
- SwissProt: Q15691 Human
- SwissProt: Q61166 Mouse
- Unigene: 472437 Human
- Unigene: 143877 Mouse
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Alternative names
- 5530600P05Rik antibody
- Adenomatous polyposis coli binding protein EB 1 antibody
- Adenomatous polyposis coli binding protein EB1 antibody
see all
Images
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Flow Cytometry - Anti-MAPRE1/EB1 antibody [KT51] (ab53358)
Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab53358 has been referenced in 20 publications.
- Almeida AC et al. Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals. Cell Rep 39:110610 (2022). PubMed: 35385739
- Zheng Y et al. Decreased tubulin-binding cofactor B was involved in the formation disorder of nascent astrocyte processes by regulating microtubule plus-end growth through binding with end-binding proteins 1 and 3 after chronic alcohol exposure. Front Cell Neurosci 16:989945 (2022). PubMed: 36385945
- Palander O et al. Nonredundant roles of DIAPHs in primary ciliogenesis. J Biol Chem 296:100680 (2021). PubMed: 33872598
- Wilson ZT et al. Delayed KCNQ1/KCNE1 assembly on the cell surface helps IKs fulfil its function as a repolarization reserve in the heart. J Physiol 599:3337-3361 (2021). PubMed: 33963564
- Umair M et al. Biallelic variant in DACH1, encoding Dachshund Homolog 1, defines a novel candidate locus for recessive postaxial polydactyly type A. Genomics 113:2495-2502 (2021). PubMed: 34022343