Recombinant Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)
![Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)](https://www.abcam.com/ps/products/168/ab168374/Images/ab168374-242091-anti-math1-hath1-antibody-epr64192-western-blot.jpg "Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6419(2)] to MATH1/HATH1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MATH1/HATH1 antibody [EPR6419(2)]
See all MATH1/HATH1 primary antibodies -
Description
Rabbit monoclonal [EPR6419(2)] to MATH1/HATH1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human MATH1/HATH1 aa 1-100.
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Positive control
- Fetal brain and HepG2 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6419(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa). |
Target
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Function
Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. May play a role in the differentiation of subsets of neural cells by activating E box-dependent transcription. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 474 Human
- Entrez Gene: 11921 Mouse
- Entrez Gene: 500156 Rat
- Omim: 601461 Human
- SwissProt: Q92858 Human
- SwissProt: P48985 Mouse
- Unigene: 532680 Human
- Unigene: 57229 Mouse
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Alternative names
- ATH1 antibody
- ATOH1 antibody
- ATOH1_HUMAN antibody
see all
Images
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Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374) at 1/1000 dilution (purified) + Human fetal brain tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)](https://www.abcam.com/ps/products/168/ab168374/Images/ab168374-242090-anti-math1-hath1-antibody-epr64192-western-blot.jpg)
Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374) at 1/2000 dilution (purified) + HepG2 cell lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)](https://www.abcam.com/ps/products/168/ab168374/Images/ab168374-242089-anti-math1-hath1-antibody-epr64192-western-blot.jpg)
Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374) at 1/1000 dilution (purified) + Rat brain tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)](https://www.abcam.com/ps/products/168/ab168374/Images/ab168374-242088-anti-math1-hath1-antibody-epr64192-western-blot.jpg)
Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374) at 1/1000 dilution (purified) + Mouse brain tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)](https://www.abcam.com/ps/products/168/ab168374/Images/ab168374-166663-anti-math1-hath1-antibody-epr64192-western-blot.jpg)
Western blot - Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)All lanes : Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374) at 1/1000 dilution (unpurified)
Lane 1 : Fetal brain lysate
Lane 2 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
![Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)](https://www.abcam.com/ps/products/168/ab168374/Images/ab168374-6-benefits-of-recombinant-antibodies.png)
Anti-MATH1/HATH1 antibody [EPR6419(2)] (ab168374)
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab168374 has been referenced in 4 publications.
- Zhang YL et al. Small activating RNA activation of ATOH1 promotes regeneration of human inner ear hair cells. Bioengineered 13:6729-6739 (2022). PubMed: 35246011
- Li J et al. Aberrant Gcm1 expression mediates Wnt/ß-catenin pathway activation in folate deficiency involved in neural tube defects. Cell Death Dis 12:234 (2021). PubMed: 33664222
- Akgül S et al. Opposing Tumor-Promoting and -Suppressive Functions of Rictor/mTORC2 Signaling in Adult Glioma and Pediatric SHH Medulloblastoma. Cell Rep 24:463-478.e5 (2018). PubMed: 29996106
- Wölnerhanssen BK et al. Deregulation of transcription factors controlling intestinal epithelial cell differentiation; a predisposing factor for reduced enteroendocrine cell number in morbidly obese individuals. Sci Rep 7:8174 (2017). PubMed: 28811552