Recombinant Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)
![Immunoprecipitation - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)](https://www.abcam.com/ps/products/151/ab151739/Images/ab151739-8-anti-matrin-3-antibody-epr10634b-immunoprecipitation-hap1-human.jpg "Immunoprecipitation - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10634(B)] to Matrin 3
- Suitable for: WB, IHC-P, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Matrin 3 antibody [EPR10634(B)]
See all Matrin 3 primary antibodies -
Description
Rabbit monoclonal [EPR10634(B)] to Matrin 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Fetal kidney, HeLa, MCF-7, and HepG2 cell lysates. IHC: Human colon and endometrial carcinoma tissue. IF: MCF-7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10634(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 95 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF | (2) |
1/100 - 1/250.
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| IP |
1/2000.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Predicted molecular weight: 95 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
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IP
1/2000. |
Target
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Function
May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. -
Involvement in disease
Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders. -
Sequence similarities
Contains 1 matrin-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus matrix. - Information by UniProt
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Database links
- Entrez Gene: 9782 Human
- Entrez Gene: 17184 Mouse
- Entrez Gene: 29150 Rat
- Omim: 164015 Human
- SwissProt: P43243 Human
- SwissProt: Q8K310 Mouse
- SwissProt: P43244 Rat
- Unigene: 268939 Human
see all -
Alternative names
- ALS21 antibody
- KIAA0723 antibody
- Matr3 antibody
see all
Images
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Immunoprecipitation - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)Immunoprecipitation of MATR3 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 2.0μg of ab151739 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab151714 at 1/2000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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![Western blot - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)](https://www.abcam.com/ps/products/151/ab151739/Images/ab151739-7-anti-matrin-3-antibody-epr10634b-western-blot-hap1-knockout-matr3-human.jpg)
Western blot - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)ab151739 was shown to react with MATR3 in wild-type HAP1 cells in Western blot with loss of signal observed in a MATR3 knockout cell line. Wild-type HAP1 and MATR3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab151739 overnight at 4 °C at a 1/10000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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![Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)](https://www.abcam.com/ps/products/151/ab151739/Images/ab151739-158829-anti-matrin-3-antibody-epr10634b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)Immunofluorescence analysis of MCF-7 cells labeling Matrin 3 with ab151739 at 1/100. -
![Western blot - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)](https://www.abcam.com/ps/products/151/ab151739/Images/ab151739-158832-anti-matrin-3-antibody-epr10634b-western-blot.jpg)
Western blot - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)All lanes : Anti-Matrin 3 antibody [EPR10634(B)] (ab151739) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 95 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)](https://www.abcam.com/ps/products/151/ab151739/Images/ab151739-158831-anti-matrin-3-antibody-epr10634b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Matrin 3 with ab151739 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)](https://www.abcam.com/ps/products/151/ab151739/Images/ab151739-158830-anti-matrin-3-antibody-epr10634b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)Immunohistochemical analysis of paraffin-embedded endometrial carcinoma labeling Matrin 3 with ab151739 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)](https://www.abcam.com/ps/products/151/ab151739/Images/ab151739-5-benefits-of-recombinant-antibodies.png)
Anti-Matrin 3 antibody [EPR10634(B)] (ab151739)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab151739 has been referenced in 2 publications.
- Pan X et al. 5'-UTR SNP of FGF13 causes translational defect and intellectual disability. Elife 10:N/A (2021). PubMed: 34184986
- Malik AM et al. Matrin 3-dependent neurotoxicity is modified by nucleic acid binding and nucleocytoplasmic localization. Elife 7:N/A (2018). ICC . PubMed: 30015619