Anti-Matrix protein 1 antibody (ab20910)
Key features and details
- Goat polyclonal to Matrix protein 1
- Suitable for: ICC/IF, ELISA
- Reacts with: Influenza A
- Isotype: IgG
Overview
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Product name
Anti-Matrix protein 1 antibody
See all Matrix protein 1 primary antibodies -
Description
Goat polyclonal to Matrix protein 1 -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, ELISAmore details
Unsuitable for: IHC -
Species reactivity
Reacts with: Influenza A -
Immunogen
Full length native protein (purified) corresponding to Matrix protein 1. Purified M1 protein, Influenza A-Phillipines (H3N2).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
>95% pure. Sodium sulfate precipitation and ion-exchange chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Influenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 1461001 Influenza A
- Entrez Gene: 956527 Influenza A
- SwissProt: P03485 Influenza A
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Alternative names
- M1 antibody
- Matrix Protein 1 antibody
- Matrix Protein I antibody
- Membrane matrix protein M1 antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Matrix protein 1 antibody (ab20910)This image is courtesy of an abreview submitted by Maria Amorim.
Immunocytochemical immunoflurescence analysis of Formaldehyde-fixed human kidney epithelial cells, labelling Influenza A Virus M1 matrix protein with ab20910 at a dilution of 1/500 incubated fro 1 hour at 18°C in 1% FCS PBS. Blocking was with 1% serum incubated for 30 minutes at 18°C. Secondary was a donkey anti-goat Alexa Fluor® 568 undiluted. Cells were transfected with either GFP-M1 or pCDNA2-NP, as indicated on the left hand side of the figure. 24h later cells were fixed and stained for M1 using ab20910 (red) or NP using ab20343 (grey). The abcam 20910 detected M1 in cells transfected with GFP-M1 but not pCDNA3-NP. Moreover, the level of co-localization between GFP-M1 and ab20910 was quite good.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab20910 has been referenced in 16 publications.
- Silvano M et al. Gene Expression Analysis of Adapted Insect Cells during Influenza VLP Production Using RNA-Sequencing. Viruses 14:N/A (2022). PubMed: 36298794
- Al-Beltagi S et al. Thapsigargin Is a Broad-Spectrum Inhibitor of Major Human Respiratory Viruses: Coronavirus, Respiratory Syncytial Virus and Influenza A Virus. Viruses 13:N/A (2021). PubMed: 33546185
- Dias MM et al. Insect High Five™ cell line development using site-specific flipase recombination technology. G3 (Bethesda) 11:N/A (2021). PubMed: 33982066
- Zheng M et al. Caspase-6 Is a Key Regulator of Innate Immunity, Inflammasome Activation, and Host Defense. Cell 181:674-687.e13 (2020). PubMed: 32298652
- Kesavardhana S et al. The Za2 domain of ZBP1 is a molecular switch regulating influenza-induced PANoptosis and perinatal lethality during development. J Biol Chem 295:8325-8330 (2020). PubMed: 32350114