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RecombinantRabMAb

Recombinant Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)

Reviews (3) Submit a question References (3)
Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
  • ChIP-sequencing - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
  • Immunoprecipitation - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
  • Immunoprecipitation - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
  • Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
  • Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
  • Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
  • Immunoprecipitation - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
  • Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19352] to MAX - ChIP Grade
  • Suitable for: ChIP-sequencing, IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-MAX antibody [EPR19352] - ChIP Grade
    See all MAX primary antibodies

  • Description

    Rabbit monoclonal [EPR19352] to MAX - ChIP Grade

  • Host species

    Rabbit

  • Specificity

    ab199489 reacted weakly in tissue lysate samples and requires further optimization.

  • Tested applications

    Suitable for: ChIP-sequencing, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, Jurkat, K562 and Raji whole cell lysates; mouse and rat brain tissue lysate; IP: mouse and rat brain tissue lysate. ChIP-seq: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab199489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP-sequencing
Use 8µg for 107 cells.
IP (1)
1/30.
WB (1)
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 18 kDa).
Notes
ChIP-sequencing
Use 8µg for 107 cells.
IP
1/30.
WB
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 18 kDa).

Target

  • Function

    Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity.

  • Tissue specificity

    High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.

  • Involvement in disease

    Pheochromocytoma

  • Sequence similarities

    Belongs to the MAX family.
    Contains 1 bHLH (basic helix-loop-helix) domain.

  • Post-translational
    modifications

    Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes.

  • Cellular localization

    Nucleus. Cell projection, dendrite.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • bHLHd4 antibody
    • bHLHd5 antibody
    • bHLHd6 antibody
    • bHLHd7 antibody
    • bHLHd8 antibody
    • Class D basic helix-loop-helix protein 4 antibody
    • Helix loop helix zipper protein antibody
    • Max antibody
    • MAX protein antibody
    • MAX_HUMAN antibody
    • MGC10775 antibody
    • MGC11225 antibody
    • MGC18164 antibody
    • MGC34679 antibody
    • MGC36767 antibody
    • MYC associated factor X antibody
    • Myc binding novel HLH/LZ protein antibody
    • Myc-associated factor X antibody
    • Orf 1 antibody
    • Orf1 antibody
    • Protein max antibody
    see all

Images

  • Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    All lanes : Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 19 kDa why is the actual band size different from the predicted?



    This blot was developed using a higher sensitivity ECL substrate.

  • ChIP-sequencing - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    ChIP-sequencing - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 8 µg of ab199489 [EPR19352]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

    Additional screenshots of mapped reads can be downloaded here.

  • Immunoprecipitation - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    Immunoprecipitation - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    MAX was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 µg with ab199489 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab199489 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10 µg

    Lane 2: ab199489 IP in Rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199489 in rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

  • Immunoprecipitation - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    Immunoprecipitation - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    MAX was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 µg with ab199489 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab199489 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 µg

    Lane 2: ab199489 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199489 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

  • Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    Lanes 1-4 : Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489) at 1/1000 dilution
    Lane 5 : Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 5 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 19 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    All lanes : Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytoplasmic fraction lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 19 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    Western blot - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/20000 dilution

    Predicted band size: 18 kDa
    Observed band size: 19 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Recognition of MAX in tissue lysates was very weak and requires optimization.

  • Immunoprecipitation - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    Immunoprecipitation - Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)

    MAX was immunoprecipitated from 0.35 mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab199489 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab199489 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: 293 whole cell lysate 10µg (Input).

    Lane 2: ab199489 IP in HEK-293 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199489 in HEK-293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

  • Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)
    Anti-MAX antibody [EPR19352] - ChIP Grade (ab199489)

References (3)

Publishing research using ab199489? Please let us know so that we can cite the reference in this datasheet.

ab199489 has been referenced in 3 publications.

  • Qu S  et al. PD-L1 lncRNA splice isoform promotes lung adenocarcinoma progression via enhancing c-Myc activity. Genome Biol 22:104 (2021). PubMed: 33849634
  • Sheng X  et al. IRE1a-XBP1s pathway promotes prostate cancer by activating c-MYC signaling. Nat Commun 10:323 (2019). PubMed: 30679434
  • Huang Y  et al. S6K1 phosphorylation-dependent degradation of Mxi1 by ß-Trcp ubiquitin ligase promotes Myc activation and radioresistance in lung cancer. Theranostics 8:1286-1300 (2018). PubMed: 29507620

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAX antibody [EPR19352] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (testis cell)
Permeabilization
No
Specification
testis cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 07 2022

Immunoprecipitation abreview for Anti-MAX antibody [EPR19352] - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (keratinocytes)
Total protein in input
2e+006 cells
Immuno-precipitation step
Protein G
Specification
keratinocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Julien Coutier

Verified customer

Submitted Sep 04 2018

Western blot abreview for Anti-MAX antibody [EPR19352] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (skin keratinocytes)
Gel Running Conditions
Reduced Denaturing (any KD from BioRad)
Loading amount
35 µg
Specification
skin keratinocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Julien Coutier

Verified customer

Submitted Feb 17 2017

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