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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

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Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
  • ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
  • Flow Cytometry (Intracellular) - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
  • ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
  • Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
  • Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9913] to MBD3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ChIP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Unconjugated

Overview

  • Product name

    Anti-MBD3 antibody [EPR9913] - BSA and Azide free
    See all MBD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9913] to MBD3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ChIP, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240144 is the carrier-free version of ab157464.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ChIP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ChIP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Application notes
Is unsuitable for IHC-P.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling MBD3 with purified ab157464 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

  • ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

    Chromatin was prepared from NIH/3T3 cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab157464 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

  • Flow Cytometry (Intracellular) - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Flow Cytometry (Intracellular) - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

    Intracellular flow cytometric analysis of permeabilized 293T cells labeling MBD3 with ab157464 at 1/10 dilution (red) compared to a rabbit IgG negative control (green). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464). 

     

     

  • ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

    Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab157464 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

  • Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Immunofluorescent analysis of HeLa cells labeling MBD3 with ab157464 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

  • Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)
    Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab240144? Please let us know so that we can cite the reference in this datasheet.

ab240144 has not yet been referenced specifically in any publications.

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