Anti-MDH2 antibody [2F5AF8] (ab110317)
![Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody [2F5AF8] (ab110317)](https://www.abcam.com/ps/products/110/ab110317/Images/ab110317-123607-anti-mdh2-antibody-2f5af8-immunofluorescence.gif "Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody [2F5AF8] (ab110317)")
Key features and details
- Mouse monoclonal [2F5AF8] to MDH2
- Suitable for: ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-MDH2 antibody [2F5AF8]
See all MDH2 primary antibodies -
Description
Mouse monoclonal [2F5AF8] to MDH2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fibroblast cells Human heart homogenate Rat liver homogenate Mouse liver homogenate HepG2 whole cell lysate (ab7900)
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
2F5AF8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ICC/IF
Use a concentration of 1 - 4 µg/ml. |
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IP
Use at an assay dependent concentration. |
Target
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Sequence similarities
Belongs to the LDH/MDH superfamily. MDH type 1 family. -
Post-translational
modificationsAcetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 4191 Human
- Entrez Gene: 17448 Mouse
- Entrez Gene: 81829 Rat
- Omim: 154100 Human
- SwissProt: P40926 Human
- SwissProt: P08249 Mouse
- SwissProt: P04636 Rat
- Unigene: 520967 Human
see all -
Alternative names
- M MDH antibody
- Malate dehydrogenase 2, NAD (mitochondrial) antibody
- Malate dehydrogenase antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody [2F5AF8] (ab110317)
Immunocytochemistry image of stained fibroblast cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the ab110317 (1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
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![Immunoprecipitation - Anti-MDH2 antibody [2F5AF8] (ab110317)](https://www.abcam.com/ps/products/110/ab110317/Images/ab110317-123606-anti-mdh2-antibody-2f5af8-immunoprecipitation.gif)
Immunoprecipitation - Anti-MDH2 antibody [2F5AF8] (ab110317)ab110317 pulls down the 33kDa MDH2 protein from Human heart homogenate (1), Rat liver homogenate (2), Mouse liver homogenate (3) and HepG2 whole cell lysate (4). The identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.
Lane 1 : Anti-MDH2 antibody [2F5AF8] (ab110317)
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Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab110317 has been referenced in 2 publications.
- Ou T et al. SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching. Clin Transl Med 10:e172 (2020). PubMed: 32997407
- Ma X et al. Dietary L-arginine supplementation affects the skeletal longissimus muscle proteome in finishing pigs. PLoS One 10:e0117294 (2015). WB ; Pig . PubMed: 25635834