Name: Anti-MEF2A + MEF2C antibody [EPR19089-34]
Brand: RabMAb
SKU: ab197070
Rating: 5 (2 reviews)

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Western blot - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19089-34] to MEF2A + MEF2C
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-MEF2A + MEF2C antibody [EPR19089-34]
See all MEF2A+MEF2C primary antibodies
Description

Rabbit monoclonal [EPR19089-34] to MEF2A + MEF2C
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Raji, Ramos, Daudi, C6 and RAW 264.7 whole cell lysates; Mouse cerebral cortex and brain lysates; Rat cerebral cortex, brain and spleen lysates; Wild-type THP-1 and Daudi cell lysates. IHC-P: Human tonsil and colonic adenocarcinoma tissues; Mouse spleen and colon tissues; Rat spleen tissue. ICC/IF: K562 and Raji cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19089-34
Isotype

IgG
Research areas
- Neuroscience
- Development

Alternative Versions
- Anti-MEF2A + MEF2C antibody [EPR19089-34] - BSA and Azide free (ab238446)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab197070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/30.
WB	(1)	1/1000. Detects a band of approximately 50-60 kDa (predicted molecular weight: 51 kDa).
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/250.

Notes
Flow Cyt (Intra) 1/30.
WB 1/1000. Detects a band of approximately 50-60 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.

Cellular localization

MEF2A: Nucleus. MEF2C: Nucleus.
Database links
- Entrez Gene: 4205 Human
- Entrez Gene: 4208 Human
- Entrez Gene: 17258 Mouse
- Entrez Gene: 17260 Mouse
- Entrez Gene: 309957 Rat
- Entrez Gene: 499497 Rat
- Omim: 600660 Human
- Omim: 600662 Human
- SwissProt: Q02078 Human
- SwissProt: Q06413 Human
- SwissProt: Q60929 Mouse
- SwissProt: Q8CFN5 Mouse
- SwissProt: Q2MJT0 Rat
- Unigene: 268675 Human
- Unigene: 649965 Human
- Unigene: 132788 Mouse
- Unigene: 24001 Mouse
- Unigene: 426509 Mouse
- Unigene: 451574 Mouse
- Unigene: 466976 Mouse
- Unigene: 484098 Mouse
- Unigene: 162435 Rat
see all

Western blot - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

All lanes : Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070) at 1/1000 dilution

Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : MEF2C knockout THP-1 cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Performed under reducing conditions.

Predicted band size: 51 kDa
Observed band size: 55/60 kDa why is the actual band size different from the predicted?

False colour image of Western blot: Anti-MEF2A + MEF2C antibody [EPR19089-34] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab197070 was shown to bind specifically to MEF2C. A band was observed at 55/60 kDa in wild-type THP-1 cell lysates with no signal observed at this size in MEF2C knockout cell line. To generate this image, wild-type and MEF2C knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
Western blot - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

All lanes : Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070) at 1/1000 dilution

Lane 1 : Recombinant Human MEF2A protein (ab152519)
Lane 2 : Human MEF2C full length protein

Lysates/proteins at 0.1 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 51 kDa
Observed band size: 55,83 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

ab197070 recognizes the full length tagged recombinant proteins MEF2A and MEF2C which have expected molecular weights of 83 and 55 kDa respectively.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197070 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Flow Cytometry (Intracellular) - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) labelling MEF2A + MEF2C with purified ab197070 at 1/30 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor^® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

All lanes : Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070) at 1/1000 dilution

Lane 1 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 50-60 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
MEF2C is expressed specifically in cells from B-lymphocyte lineage but not T cell lineage. Jurkat cell lysate serves as a negative control (PMID: 9798649).
The 50-60KD bands observed are consistent with what has been described in the literature (PMID: 18450586)
Western blot - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

All lanes : Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070) at 1/5000 dilution

Lane 1 : Mouse cerebral cortex lysate
Lane 2 : Rat cerebral cortex lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 50-60 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
The 50-60KD bands observed are consistent with what has been described in the literature (PMID: 18450586)
Western blot - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

All lanes : Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 50-60 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1minute; Lane 2 and 3: 3minutes.
The 50-60KD bands observed are consistent with what has been described in the literature (PMID: 18450586).
Western blot - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

All lanes : Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 50-60 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2: 30 seconds.
The 50-60KD bands observed are consistent with what has been described in the literature (PMID: 18450586).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on B lymphocytes of the Human tonsil is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on lymphocytes of the colonic adenocarcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on normal Human thymus is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on B lymphocytes of the mouse spleen, the T cells in the periarterial lymphatic sheath showed negative staining. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on the mouse colon epithelium, the lymphocytes on the interstitial substance showed nuclear staining. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on B lymphocytes of the rat spleen, the T cells in the periarterial lymphatic sheath showed negative staining. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling MEF2C with ab197070 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat testis is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling MEF2C with ab197070 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on K562 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab197070 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (Human Burkitt's lymphoma cell line) cells labeling MEF2C with ab197070 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Raji cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab197070 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (Human Burkitt's lymphoma cell line) and Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MEF2C with ab197070 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Raji cell line. Negative expression in Jurkat cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab197070 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Anti-MEF2A + MEF2C antibody [EPR19089-34] (ab197070)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab197070? Please let us know so that we can cite the reference in this datasheet.

ab197070 has been referenced in 10 publications.

Bartosova L et al. Quercetin alleviates diastolic dysfunction and suppresses adverse pro-hypertrophic signaling in diabetic rats. Front Endocrinol (Lausanne) 13:1029750 (2022). PubMed: 36568083
Pronobis MI et al. In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration. Elife 10:N/A (2021). PubMed: 33764296
Honkoop H et al. Live imaging of adult zebrafish cardiomyocyte proliferation ex vivo. Development 148:N/A (2021). PubMed: 34397091
Nath SR et al. MEF2 impairment underlies skeletal muscle atrophy in polyglutamine disease. Acta Neuropathol 140:63-80 (2020). PubMed: 32306066
Shoffner A et al. Tp53 Suppression Promotes Cardiomyocyte Proliferation during Zebrafish Heart Regeneration. Cell Rep 32:108089 (2020). PubMed: 32877671
Bjorness TE et al. An essential role for MEF2C in the cortical response to loss of sleep in mice. Elife 9:N/A (2020). PubMed: 32851972
Saito H et al. TG-interacting factor 1 (Tgif1)-deficiency attenuates bone remodeling and blunts the anabolic response to parathyroid hormone. Nat Commun 10:1354 (2019). PubMed: 30902975
Miyamoto K et al. Direct In Vivo Reprogramming with Sendai Virus Vectors Improves Cardiac Function after Myocardial Infarction. Cell Stem Cell 22:91-103.e5 (2018). PubMed: 29276141
Cheng Z et al. The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway. Exp Mol Med 50:e447 (2018). WB . PubMed: 29504607
Harrington AJ et al. MEF2C regulates cortical inhibitory and excitatory synapses and behaviors relevant to neurodevelopmental disorders. Elife 5:N/A (2016). WB, IHC (PFA fixed) ; Mouse . PubMed: 27779093

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1-2 of 2 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Embryonic Heart)

Permeabilization

Yes - Tween-20

Specification

Embryonic Heart

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 06 2016

Application

Western blot

Sample

Mouse Tissue lysate - whole (Heart)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

10 µg

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 20 2016

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Cellular localization

Database links

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (10)

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-MEF2A + MEF2C antibody [EPR19089]

Western blot abreview for Anti-MEF2A + MEF2C antibody [EPR19089]