Name: Anti-MelanA antibody [EPR20380]
Brand: RabMAb
SKU: ab210546
Rating: 5 (15 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [EPR20380] (ab210546)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20380] to MelanA
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
Reacts with: Mouse, Human

Alexa Fluor® 647 APC Carrier Free PE

Product name

Anti-MelanA antibody [EPR20380]
See all MelanA primary antibodies
Description

Rabbit monoclonal [EPR20380] to MelanA
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
Species reactivity

Reacts with: Mouse, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human melanoma lysate; B16F0 and MeWo whole cell lysates. IHC-P: Human malignant melanoma and metastatic malignant melanoma tissues; Mouse hair bulb tissue. ICC/IF: MeWo and B16F0 cells. Flow Cyt (intra): B16F0 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20380
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human MelanA protein (ab114312)
- Recombinant Human MelanA protein (ab157272)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P	(8)	1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB	(1)	1/2000. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
ICC/IF	(1)	1/1000.
Flow Cyt (Intra)		1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
ICC/IF 1/1000.
Flow Cyt (Intra) 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Function

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Tissue specificity

Expression is restricted to melanoma and melanocyte cell lines and retina.
Post-translational
modifications

Acylated.
Cellular localization

Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
Target information above from: UniProt accession Q16655 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2315 Human
- Entrez Gene: 77836 Mouse
- Omim: 605513 Human
- SwissProt: Q16655 Human
- Unigene: 154069 Human
Alternative names
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
- Antigen SK29-AA antibody
- MAR1_HUMAN antibody
- MART 1 antibody
- MART-1 antibody
- MART1 antibody
- Melan A antibody
- Melan A protein antibody
- Melanoma antigen recognized by T cells 1 antibody
- Melanoma antigen recognized by T-cells 1 antibody
- MLAN A antibody
- MLANA antibody
- OTTHUMP00000021036 antibody
- OTTHUMP00000021037 antibody
- OTTHUMP00000021038 antibody
- Protein Melan-A antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [EPR20380] (ab210546)

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue labeling MelanA with ab210546 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on human malignant melanoma is observed [PMID: 17445277] [PMID: 21840568].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MelanA antibody [EPR20380] (ab210546)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MeWo (Human malignant melanoma cell line) cells labeling MelanA with ab210546 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on MeWo cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MelanA antibody [EPR20380] (ab210546)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized B16F0 (Mouse melanoma cell line) cells labeling MelanA with ab210546 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on B16F0 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [EPR20380] (ab210546)

Immunohistochemical analysis of paraffin-embedded human metastatic malignant melanoma tissue labeling MelanA with ab210546 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on human metastatic malignant melanoma is observed [PMID: 17445277] [PMID: 21840568].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [EPR20380] (ab210546)

Immunohistochemical analysis of paraffin-embedded mouse hair bulb tissue labeling MelanA with ab210546 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on melanocytes of mouse hair bulb is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-MelanA antibody [EPR20380] (ab210546)

All lanes : Anti-MelanA antibody [EPR20380] (ab210546) at 1/2000 dilution

Lane 1 : Human melanoma lysate
Lane 2 : A-375 (Human malignant melanoma cell line) whole cell lysate
Lane 3 : B16F0 (Mouse melanoma cell line) whole cell lysate
Lane 4 : MeWo (Human malignant melanoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 13 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1 and 2: 10 seconds; Lane 3 and 4: 3 seconds.

A-375 doesn’t express MelanA. The expression profile observed is consistent with what has been described in the literature (PMID: 12670905).
Flow Cytometry (Intracellular) - Anti-MelanA antibody [EPR20380] (ab210546)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed B16F0 (Mouse melanoma cell line) cells labeling MelanA with ab210546 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [EPR20380] (ab210546)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling MelanA with ab210546 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: no staining on human liver cancer [PMID: 10096358].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-MelanA antibody [EPR20380] (ab210546)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab210546? Please let us know so that we can cite the reference in this datasheet.

ab210546 has been referenced in 13 publications.

Morita D et al. Effects of ultraviolet irradiation with a LED device on bone metabolism associated with vitamin D deficiency in senescence-accelerated mouse P6. Heliyon 6:e03499 (2020). PubMed: 32140604
Makida K et al. Low energy irradiation of narrow-range UV-LED prevents osteosarcopenia associated with vitamin D deficiency in senescence-accelerated mouse prone 6. Sci Rep 10:11892 (2020). PubMed: 32681041
Maaßen T et al. Monosomy-3 Alters the Expression Profile of the Glucose Transporters GLUT1-3 in Uveal Melanoma. Int J Mol Sci 21:N/A (2020). PubMed: 33302435
Polisetti N et al. Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker. Sci Rep 10:17588 (2020). PubMed: 33067486
Jiang Y et al. Functional and aesthetic reconstruction of digital flexion contractures with full-thickness plantar skin grafts in children. Dermatol Ther 33:e14466 (2020). PubMed: 33112495

View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-10 of 15 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Popliteal lymph nodes with melanoma cell invasion,)

Permeabilization

Yes - 0.1% Triton X100 in PBS

Specification

Popliteal lymph nodes with melanoma cell invasion,

Blocking step

1% BSA + 5% Goat serum in PBS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 11 2022

Application

Western blot

Sample

Human Cell lysate - whole cell (Melanoma cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

Melanoma cells

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 10 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue sections (Melanoma suspicious cutaneous tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA

Permeabilization

Yes - H2O2

Specification

Melanoma suspicious cutaneous tissue

Blocking step

No blocking step used for 32 minute(s) · Temperature: 20°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 17 2021

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Melanoma)

Permeabilization

Specification

Melanoma

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 02 2021

Application

Immunocytochemistry

Sample

Human Cultured Cells (Melanoma cells)

Specification

Melanoma cells

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 15 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Melanoma)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA

Permeabilization

Specification

Melanoma

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 04 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Melanoma cells)

Specification

Melanoma cells

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 27 2021

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Melanoma)

Permeabilization

Specification

Melanoma

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 10 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (mouse skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: cirtric acid

Permeabilization

Specification

mouse skin

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 09 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (melanoma xenograft)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

melanoma xenograft

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C

Fixative

10% NBF

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 06 2019

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (13)

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-MelanA antibody [EPR20380]

Western blot abreview for Anti-MelanA antibody [EPR20380]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MelanA antibody [EPR20380]

Immunohistochemistry (Frozen sections) abreview for Anti-MelanA antibody [EPR20380]

Immunocytochemistry abreview for Anti-MelanA antibody [EPR20380]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MelanA antibody [EPR20380]

Immunocytochemistry/ Immunofluorescence abreview for Anti-MelanA antibody [EPR20380]

Immunohistochemistry (Frozen sections) abreview for Anti-MelanA antibody [EPR20380]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MelanA antibody [EPR20380]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MelanA antibody [EPR20380]

Post-translational
modifications